Antibodies against E-cadherin, vimentin, fibronectin, ZO-1, Fra-1, Ku80 and GAPDH were from Santa Cruz Biotechnology (Santa Cruz, CA). The ZEB1, Snail, Slug, ERK1/2, p-ERK1/2, AKT, p-AKT, STAT3, p-STAT3 (Y705) and ac-STAT3 (K685) antibodies were from Cell Signaling Technology (Beverly, MA). H3 and c-Fos antibodies were from Abcam (Cambridge, UK). The siRNAs targeting STAT3, Fra-1 and c-Fos were from Santa Cruz. All the chemical inhibitors were from Calbiochem (San Diego, CA).
Ac stat3 k685
Manufactured by Cell Signaling Technology
Ac-STAT3 (K685) is a recombinant protein that corresponds to the acetylated form of the transcription factor STAT3 at lysine 685. It is a tool used for research purposes.
Automatically generated - may contain errors
Lab products found in correlation
Erk1 2, by Cell Signaling Technology (1 mentions)
P akt, by Cell Signaling Technology (1 mentions)
P erk1 2, by Cell Signaling Technology (1 mentions)
Stat3, by Cell Signaling Technology (1 mentions)
Fibronectin, by Santa Cruz Biotechnology (1 mentions)
Vimentin, by Santa Cruz Biotechnology (1 mentions)
Fra 1, by Santa Cruz Biotechnology (1 mentions)
P stat3 y705, by Cell Signaling Technology (1 mentions)
Snail, by Cell Signaling Technology (1 mentions)
E cadherin, by Santa Cruz Biotechnology (1 mentions)
Gapdh, by Santa Cruz Biotechnology (1 mentions)
Radio immunoprecipitation assay ripa buffer, by Beyotime (1 mentions)
P stat3 tyr705, by Cell Signaling Technology (1 mentions)
Bca kit, by Beyotime (1 mentions)
Polyvinylidene fluoride (pvdf), by Merck Group (1 mentions)
E cadherin, by Cell Signaling Technology (1 mentions)
β actin, by Beyotime (1 mentions)
N cadherin, by Cell Signaling Technology (1 mentions)
Vimentin, by Cell Signaling Technology (1 mentions)
Gapdh, by Beyotime (1 mentions)
2 protocols using ac stat3 k685
1
Epithelial-Mesenchymal Transition Regulation
2
Protein Extraction and Western Blot Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total protein was extracted using Radio-Immunoprecipitation Assay (RIPA) buffer (Beyotime Co. Ltd). A bicinchoninic acid (BCA) kit (Beyotime Co. Ltd) was used to measure protein concentration. Proteins (20 µg) were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by transfer to polyvinylidene uoride membranes (PVDF; Millipore, Billerica, MA, USA). Antibodies used were RECK, Mortalin, E-cadherin, N-cadherin, p-STAT3 Tyr705 , Ac-STAT3 K685 and vimentin (Cell Signaling Technology, 1: 1000), GAPDH and β-actin (Beyotime Co. Ltd, 1: 500) (Supplementary Table S2).
Densitometric analysis was performed using Image-Pro-Plus 6.0 software, and GAPDH or β-actin served as the internal controls to correct for differences in protein loading.
Densitometric analysis was performed using Image-Pro-Plus 6.0 software, and GAPDH or β-actin served as the internal controls to correct for differences in protein loading.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!