Microcrystalline cellulose (MCC), PEG and dialysis bags, molecular weight 800–14,000, were purchased from Sinopharm Chemical Reagent (sinoreagent.com , accessed on 1 August 2022); Sulfuric acid, 98% concentration, and Glycerol, analytical Pure, were provided by Beijing Chemical Reagent (bjhgjt.com.cn , accessed on 1 August 2022).
Dialysis bag
Manufactured by Sinopharm
Sourced in China
A dialysis bag is a permeable membrane-based laboratory equipment used for the separation and purification of molecules based on their size or molecular weight. It functions by allowing the selective passage of certain molecules through the membrane while retaining others, enabling the isolation and concentration of desired substances.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Maclin Biochemical Technology (1 mentions)
Hoechst 33342, by Beyotime (1 mentions)
Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions)
Trisodium citrate, by Maclin Biochemical Technology (1 mentions)
Dulbecco s phosphate buffered saline dpbs, by Thermo Fisher Scientific (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt dimethyl sulfoxide dmso, by Merck Group (1 mentions)
Penicillin streptomycin solution, by Thermo Fisher Scientific (1 mentions)
Triethylamine tea, by Sinopharm (1 mentions)
6 protocols using dialysis bag
1
Microcrystalline Cellulose Extraction Protocol
2
Cytotoxicity and ROS Evaluation of Cur
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Cur (Mw = 368.38, purity ≥98%), sodium hydroxide, and trisodium citrate were purchased from Macklin Biochemical Co., Ltd. (Shanghai, China). The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was provided by Sigma-Aldrich Co. (St. Louis, MO). The dialysis bags were obtained from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). Dulbecco’s modified Eagle medium (DMEM), penicillin–streptomycin, and fetal bovine serum (FBS) was purchased from Gibco BRL (Gaithersburg, MD). ROS detection kit was obtained from Bio Lebo Technology Co., Ltd. (Beijing, China). Hoechst 33342 was purchased from Beyotime Biotechnology (Nanjing, China). The water used in the experiments was deionized. Other reagents were of analytic grade. MDA-MB-231 and 4T1 were obtained from the American Type Culture Collection (ATCC) (Manassas, VA). Female Balb/c mice (4–5 weeks old) were obtained from the Laboratory Animal Center of Nantong University.
3
Carboxymethyl Chitosan Conjugation Protocol
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CMCH was supplied by Zhejiang Yuhuan Biochemical Co. Ltd (China). Its carboxymethylation substitutive degree was 0.65 and the average molecular weight was 3.0 × 105 g/mol. It was purified before use by dissolution in deionized water and precipitation in acetone (two times), followed by extraction in a Soxhlet apparatus by refluxing in acetone for 24 h and drying at 60 °C under vacuum for 48 h. 1ʹ-(2-hydroxyethyl)-3,3ʹ-dimethyl-6-nitrospiro[2 H-1-benzopyran-2,2ʹ-indoline] (SP-OH, >93%, TCI); N,N-dimethylaminopyridine (DMAP, ≥99%, Sinopharm Chemical Reagent Co., Ltd); 1,3-dicyclohexylcarbodiimide (DCC, ≥99%, Sinopharm Chemical Reagent Co., Ltd); Dialysis bag (molecular weight cut-off of 8000–14,000 g/mol, Sinopharm Chemical Reagent Co., Ltd). All other chemicals were analytical reagents and used as supplied by the company.
4
In vitro F96 Release from Liposomes
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F96 release from liposomes was investigated in vitro using dialysis membranes. Briefly, 4 mL of liposomes containing about 1 mg mL−1 F96 were placed in a dialysis bag (MWCO approximately 14 000, Sinopharm Chemical Reagent Co. Ltd, China). The dialysis bag was placed in a flask containing 100 mL release medium (PBS, pH 7.4, containing 0.2% Tween-80), and placed in a water bath shaking at 100 rpm at 37 °C. At predetermined time intervals, 1 mL of the release medium was withdrawn and replaced with an equal volume of fresh medium. The concentration of F96 in the medium was determined by high-performance liquid chromatographic (HPLC) and used to calculate the cumulative release percentage of F96.
5
In Vitro Drug Release Kinetics
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HA/CNPs were collected and then suspended in 3 ml PBS containing 1% sodium dodecyl sulfate (SDS) (0.2 mol/l, pH 6.6) to adjust the drug concentration to 0.4 mg/ml. The final solution was put in a dialysis bag (Sinopharm Chemical Reagent Co., Ltd., Shanghai, China) (Mr2000). The dialysis bag was then placed in a beaker with 30 ml PBS and incubated in a water bath at 37°C, while shaking at 60 × g. A total of 1 ml of the solution was collected after 1, 2, 4, 8, 12, 18, 24, 36, 48, 60 and 72 h (isothermal medium was appended), and the OD values at 430 nm were measured. These values were drawn into a standard curve and used to calculate related concentrations and total release rates of the samples in two groups at each time point.
6
Synthesis and Characterization of PEG-Ibuprofen Conjugate
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MMC (mitomycin C, purity grade = 99.5%) was obtained from Livzon Pharmaceutical Group Co., Ltd. (Guangdong, China). 2-(4-Isobutylphenyl) propionic acid (ibuprofen, >98%), Fmoc-Lys(Boc)-OH, N,N′-dicyclohexylcarbodiimide (DCC), 4′-aminoacetophenone and 4-dimethylaminopyridine (DMAP) were purchased from Aladdin (Shanghai, China). Dialysis bag (Mw = 8000 to 12 000 Da), dichloromethane (DCM), trifluoroacetic acid (TFA) and triethylamine (TEA) were obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Dimethyl sulfoxide (DMSO), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) were all provided by Sigma-Aldrich (MO, USA). Poly(ethylene glycol) methyl ether (MeO-PEG-OH, MW = 2000 kDa) were ordered from TCI (PA, USA). Roswell Park Memorial Institute (RPMI) 1640 Medium, fetal bovine serum (FBS), penicillin-streptomycin solution, Dulbecco's phosphate-buffered saline (DPBS) and trypsin–EDTA (0.25%, w/v) solution were purchased from Invitrogen (NY, USA). All solvents used in this study were HPLC grade.
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