Ultrahydrogel dp column and guard column

The size distribution of fructans at each stream for experimental design was analyzed by size exclusion chromatography HPLC-SEC, using a 1220 Infinity LC System for HPLC coupled with a refractive index detector (Agilent, Alpharetta, GA, USA) and an Ultrahydrogel DP column and guard column (7.8 mm d.i. × 300 mm, Waters, Milford, MA, USA) in the stationary phase, according to the methodology proposed by Moreno-Vilet et al. [5 (link)]. This technique allows obtaining a relative abundance of Fc, FOS and MD with DP 1–2 as glucose, fructose and sucrose.

The size distribution of fructans at initial, retentate and permeate streams was analyzed by HPLC–size exclusion chromatography (SEC), using a 1220 Infinity LC System for HPLC coupled with a refractive index detector (Agilent, Alpharetta, GA, USA) and an Ultrahydrogel DP column and guard column (7.8 mm d.i. × 300 mm, Waters, Milford, MA, USA) in the stationary phase, according to the methodology proposed by Moreno-Vilet et al. (2017) [44 (link)]. This technique allowed us to obtain a relative abundance of fructans separated in three groups or fractions for practical purposes: fructans with DP ˃ 10 (Fc), FOS with DP between 3 and 10 and mono-disaccharides (MD) with DP of 1–2, such as glucose, fructose and sucrose.