Mouse anti ha epitope igg

Manufactured by Santa Cruz Biotechnology

The Mouse anti-HA-epitope IgG is a primary antibody that binds to the HA (Hemagglutinin) epitope tag. The HA epitope tag is a commonly used protein tag that allows for the detection and purification of recombinant proteins. This antibody can be used to detect and immunoprecipitate HA-tagged proteins in various applications, such as Western blotting, immunoprecipitation, and immunofluorescence.

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Lab products found in correlation

Anti rabbit cy3, by Merck Group (2 mentions) Orca charge coupled device camera, by Hamamatsu Photonics (2 mentions) Anti mouse oregon green, by Thermo Fisher Scientific (2 mentions) Dmire2 microscope, by Leica (2 mentions) Metamorph software, by Universal Imaging (2 mentions)

Spelling variants of this product

Anti-HA (247 citations) Mouse anti-HA (45 citations) IgG mouse (6 citations) Mouse anti-HA IgG (3 citations) Mouse IgGs (3 citations) Mouse anti-IgG (2 citations)

2 protocols using mouse anti ha epitope igg

Immunofluorescence Analysis of Cellular Components

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunofluorescence analysis (IFA) was as described 64 (link). Antibodies for IFA were used at the following
dilutions: mouse anti-HA-epitope IgG (Santa Cruz Biotechnology Inc.) at 1:1000,
rabbit anti-Rab11 at 1:200 44 (link), rabbit
anti-Rab5a at 1:200 68 (link), mouse anti-p67 at
1:1000 43 (link), mouse anti-BiP at 1:10 000
41 (link), rabbit anti-VSG221 at 1:1000
68 (link), rabbit anti-RabX2 at 1:50 71 (link), rabbit anti-IGP48 (this study) at 1:50.
Secondary antibodies were used at the following dilutions: anti-mouse Oregon
Green (Molecular Probes) at 1:1000 and anti-rabbit Cy3 (Sigma) at 1:1000. Cells
were examined on a Nikon Eclipse E600 epifluorescence microscope fitted with
optically matched filter blocks and a Hamamatsu ORCA charge-coupled-device
camera. Digital images were captured using Metamorph software (Universal Imaging
Corp.) on a computer running the Windows XP operating system (Microsoft Inc.)
and the raw images processed using Photoshop CS6 software (Adobe Systems Inc.).
Confocal z-sections were acquired using a Leica DMIRE2 microscope and
deconvolved using Huygens Professional software.

Allison H., O’Reilly A.J., Sternberg J, & Field M.C. (2014). An extensive endoplasmic reticulum-localised glycoprotein family in trypanosomatids. Microbial Cell, 1(10), 325-345.

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Immunofluorescence Analysis of Cellular Compartments

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunofluorescence analysis (IFA) was as described (43 (link)). Antibodies for IFA were used at the following dilutions: mouse anti-HA-epitope IgG (Santa Cruz Biotechnology Inc.) at 1:1000, rabbit anti-Rab11 at 1:200 (54 (link)), rabbit anti-Rab5a at 1:200 (55 (link)), mouse anti-p67 at 1:1000 (56 (link)), mouse anti-BiP at 1:10 000 (57 (link)), rabbit anti-VSG221 at 1:1000 (55 (link)), rabbit anti-RabX2 at 1:50 (58 (link)), rabbit anti-IGP48 (this study) at 1:50. Secondary antibodies were used at the following dilutions: anti-mouse Oregon Green (Molecular Probes) at 1:1000 and anti-rabbit Cy3 (Sigma) at 1:1000. Cells were examined on a Nikon Eclipse E600 epifluorescence microscope fitted with optically matched filter blocks and a Hamamatsu ORCA charge-coupled-device camera. Digital images were captured using Metamorph software (Universal Imaging Corp.) on a computer running the Windows XP operating system (Microsoft Inc.) and the raw images processed using Photoshop CS6 software (Adobe Systems Inc.). Confocal z-sections were acquired using a Leica DMIRE2 microscope and deconvolved using Huygens Professional software.

Allison H., O’Reilly A.J., Sternberg J, & Field M.C. (2014). An extensive endoplasmic reticulum-localised glycoprotein family in trypanosomatids. Microbial Cell, 1(10), 325-345.

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