Plasma leptin concentrations were measured from plasma following an overnight fast (16 hours), after 4 hours of re-feeding, and subsequent to injections of recombinant mouse leptin in fasted animals. Blood was collected in heparinized tubes and immediately placed on ice then centrifuged at 2,000 RCF for 15 minutes at 4 degrees Celsius. The R&D systems Mouse/Rat Leptin Quantikine ELISA Kit (SMOB00) was used to measure plasma leptin concentrations. Statistical analysis was performed using Prism 7 from GraphPad Software, Inc. Variance is reported as the standard error of the mean.
Mouse rat leptin quantikine elisa kit smob00
Manufactured by R&D Systems
The Mouse/Rat Leptin Quantikine ELISA Kit (SMOB00) is a quantitative sandwich enzyme immunoassay designed to measure mouse and rat leptin levels in cell culture supernates, serum, and plasma. The kit utilizes a polyclonal antibody specific for mouse and rat leptin that has been pre-coated onto a microplate.
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2 protocols using mouse rat leptin quantikine elisa kit smob00
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Plasma Leptin Concentration Dynamics
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Plasma Leptin Concentration Dynamics
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Plasma leptin concentrations were measured from plasma following an overnight fast (16 hours), after 4 hours of re-feeding, and subsequent to injections of recombinant mouse leptin in fasted animals. Blood was collected in heparinized tubes and immediately placed on ice then centrifuged at 2,000 RCF for 15 minutes at 4 degrees Celsius. The R&D systems Mouse/Rat Leptin Quantikine ELISA Kit (SMOB00) was used to measure plasma leptin concentrations. Statistical analysis was performed using Prism 7 from GraphPad Software, Inc. Variance is reported as the standard error of the mean.
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