Syntaxin 1

Manufactured by Santa Cruz Biotechnology

Syntaxin-1 is a membrane-associated protein involved in the regulation of vesicle trafficking and neurotransmitter release. It is a core component of the SNARE complex, which mediates the fusion of synaptic vesicles with the presynaptic membrane.

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Lab products found in correlation

Munc18 1, by BD (1 mentions) β actin, by Merck Group (1 mentions) Rhodopsin, by Thermo Fisher Scientific (1 mentions) Lsm 510 exciter microscope, by Zeiss (1 mentions) Cyclind3, by Thermo Fisher Scientific (1 mentions) Phosphohistone h 3 ph 3, by Merck Group (1 mentions) Huc d, by Thermo Fisher Scientific (1 mentions) Neuronal nuclei (neun), by Merck Group (1 mentions) P27kip1, by BD (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)

2 protocols using syntaxin 1

Immunoblotting Protocol for Neuronal Proteins

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β‐actin (A1978, Sigma, 1:1,000), GAPDH (DSHB‐hGAPDH‐2G7, DSHB, 1:500; G‐9, Santa Cruz, 1:1,000), GFP (632381, Takara Bio Clontech, 1:2,000), Munc18‐1 (610337, BD Biosciences, 1:1,000), myc (9E10, DSHB or C3956, Sigma, 1:500), synaptotagmin‐1 (105221, Synaptic Systems, 1:50), Syntaxin‐1 (HPC‐1, Santa Cruz, 1:1,000). The monoclonal antibodies GAPDH (DSHB‐hGAPDH‐2G7) and myc (9E10) developed by DSHB and J.M. Bishop, respectively, were obtained from the Developmental Studies Hybridoma Bank, created by the NICHD of the NIH and maintained at The University of Iowa, Department of Biology, Iowa City, IA 52242.

Abramov D., Guiberson N.G., Daab A., Na Y., Petsko G.A., Sharma M, & Burré J. (2020). Targeted stabilization of Munc18‐1 function via pharmacological chaperones. EMBO Molecular Medicine, 13(1), e12354.

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Immunofluorescence Analysis of Mouse Retina

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Retinas were fixed with 4% paraformaldehyde in PBS for 30 min at room temperature, and sectioned at the thickness of 20 μm. Sample slides were washed with PBS before incubation with a blocking buffer containing 5% normal donkey serum, 0.1% Triton X-100, and 0.1% NaN₃ in PBS for 2 hours at room temperature. Primary antibodies were added for overnight incubation at 4°C. Primary anti bodies used: CyclinD3 (Thermo Scientific), Rhodopsin (Thermo Scientific), NeuN (Millipore), p27^kip1 (BD Transduction Laboratories), GS (Millipore), Ki67 (Thermo Scientific), phospho-histone H3 (PH3) (Millipore), HuC/D (Thermo Scientific), Pax6 (BioLegend), and Syntaxin1 (Santa Cruz). Sections were washed with PBS and incubated with secondary antibodies (Jackson ImmunoResearch) for 2 hours at room temperature. Cell nuclei were counterstained with DAPI (Sigma). Confocal Images were acquired using a Zeiss LSM 510 EXCITER microscope.

Yao K., Qiu S., Tian L., Snider W.D., Flannery J.G., Schaffer D.V, & Chen B. (2016). Wnt regulates proliferation and neurogenic potential of Müller glial cells through a Lin28/let-7 miRNA-dependent pathway in adult mammalian retina. Cell reports, 17(1), 165-178.

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