To determine the sex of sampled individuals, we relied on molecular sexing method94 (link). DNA was isolated with Bio-Trace DNA Purification Kit (EURx, Gdańsk, Poland, according to the kit protocol). We successfully used P2/P8 primers designed for CHD-based sex identification in non-ratite birds95 (link). The products of PCR were separated by 2% agarose gel electrophoresis, allowing to recognize females with two bands and males with one band. The sex ratio within our sample did not deviate from parity (N females = 59, N males = 61, G = 0.033, P = 0.86).
Bio trace dna purification kit
Manufactured by EURx
Sourced in Poland
The Bio-Trace DNA Purification Kit is a laboratory product designed for the extraction and purification of DNA from biological samples. The kit employs a silica-based membrane technology to capture and isolate DNA, enabling its subsequent use in various downstream applications.
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2 protocols using bio trace dna purification kit
1
Molecular Sexing of Bird Samples
2
Insect Bacteriome DNA Extraction and Sequencing
Check if the same lab product or an alternative is used in the 5 most similar protocols
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DNA was extracted from dissected bacteriomes or insect abdomens using one of three different DNA extraction kits: Sherlock AX isolation kit (A&A Biotechnology, Poland), Bio-Trace DNA Purification Kit (Eurx, Poland), and Genomic Mini AX Yeast Spin kit (A&A Biotechnology, Poland), according to manufacturers’ protocols. The metagenomic libraries for high-throughput sequencing on the Illumina platform were prepared using NEBNext Ultra II FS DNA Library Prep and NEBNext DNA Ultra II kits, with a target insert length of 350 bp. The pooled libraries were sequenced on Illumina HiSeq X or NovaSeq 6000 S4 lanes (2 × 150 bp reads).
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