Platinum sybr green protocol

Manufactured by Thermo Fisher Scientific

The Platinum SYBR Green protocol is a lab equipment product designed for real-time PCR applications. It provides a reliable and sensitive method for gene expression analysis and quantification.

Automatically generated - may contain errors

Lab products found in correlation

Trizol reagent, by Thermo Fisher Scientific (3 mentions) Genomicprep mini spin kit, by GE Healthcare (2 mentions) Cxcl10, by GE Healthcare (2 mentions) 7500 real time pcr system, by Thermo Fisher Scientific (1 mentions)

3 protocols using platinum sybr green protocol

Pulmonary Inflammation and Parasite Load Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

RNA was extracted from lungs of control and infected mice with Trizol reagent (Invitrogen), transcribed to cDNA, and primers for CCL2 (F 5-TGGCTCAGCCAGATGCAGT-3, R 5-TTGGGATCATCTTGCTGGTG-3), CCL4 (F 5-TCTTGCTCGTGGCTGCCT-3, R 5-GGGAGGGTCAGAGCCCA-3), CCL5 (F 5-CAAGTGCTCCAATCTTGCAGTC-3, R 5-TTCTCTGGGTTGGCACACAC), CCL17 (F 5-CAGGGATGCCATCGTGTTTC-3, R 5-CACCAATCTGATGGCCTTCTT-3), CCL22 (F 5-TACATCCGTCACCCTCTGCC-3, R 5-CGGTTATCAAAACAACGCCAG-3), CXCL9 (F 5-AATGCACGATGCTCCTGCA-3, R 5-GGTCTTTGAGGGATTTGTAGTG-3) and CXCL10 (F 5-GCCGTCATTTTCTGCCTCA-3, R 5-CGTCCTTGCGAGAGGGATC-3) (Integrated DNA Technologies) used to quantify gene expression by PCR.
DNA from lungs of PbN-infected mice was extracted by illustra tissue & cells genomicPrep Mini spin kit (GE Healthcare). The primers of mouse endogenous gene, GAPDH (Forward: GGCAAATTCAACGGCACAGT and Reverse: AGATGGTGATGGGCTTCCC) and the Plasmodium berghei 18 s ribosomal 18 s gene (Forward: AAGCATTAAATAAAGCGAATACATCCTTA and 18 s Reverse: GGAGATTGGTTTTGACGTTTATGT) were used to determine the parasite load in the lungs by relative quantification, normalized with the mouse GAPDH (∆CT). The quantitative PCRs were carried out with the Platinum SYBR Green protocol (Invitrogen) on an Applied Biosystems 7500 real-time PCR system.

Galvão-Filho B., de Castro J.T., Figueiredo M.M., Rosmaninho C.G., Antonelli L.R, & Gazzinelli R.T. (2019). The emergence of pathogenic TNF/iNOS producing dendritic cells (Tip-DCs) in a malaria model of acute respiratory distress syndrome (ARDS) is dependent on CCR4. Mucosal Immunology, 12(2), 312-322.

+ Open protocol

+ Expand

Quantifying Liver Transcripts in Infected Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total RNA from livers of non-infected (Control), 3 day-, 7 day- or 11 day-infected mice were isolated by TRIzol Reagent (Invitrogen). Quantitative PCR was carried out with Platinum SYBR Green protocol (Invitrogen) on an Applied Biosystems 7500 Real Time PCR System using primers listed in Table S4. Relative quantification was performed using standard curve analysis and the expression data were presented as mean of normalized expression to the GAPDH level of three biological replications.

Rocha B.C., Marques P.E., Leoratti F.M., Junqueira C., Pereira D.B., Antonelli L.R., Menezes G.B., Golenbock D.T, & Gazzinelli R.T. (2015). Type I interferon transcriptional signature in neutrophils and high frequency of low-density granulocytes are associated with tissue damage in malaria. Cell reports, 13(12), 2829-2841.

+ Open protocol

+ Expand

Gene Expression and Parasite Load Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

RNA was extracted from lungs of control and infected mice with Trizol reagent (Invitrogen), transcribed to cDNA, and primers for CCL2 (F 5-TGGCTCAGCCAGATGCAGT-3, R 5-TTGGGATCATCTTGCTGGTG-3), CCL4 (F 5-TCTTGCTCGTGGCTGCCT-3, R 5-GGGAGGGTCAGAGCCCA-3), CCL5 (F 5-CAAGTGCTCCAATCTTGCAGTC-3, R 5-TTCTCTGGGTTGGCACACAC), CCL17 (F 5-CAGGGATGCCATCGTGTTTC-3, R 5-CACCAATCTGATGGCCTTCTT-3), CCL22 (F 5-TACATCCGTCACCCTCTGCC-3, R 5-CGGTTATCAAAACAACGCCAG-3), CXCL9 (F 5-AATGCACGATGCTCCTGCA- 3, R 5-GGTCTTTGAGGGATTTGTAGTG-3) and CXCL10 (F 5- GCCGTCATTTTCTGCCTCA-3, R 5-CGTCCTTGCGAGAGGGATC-3) (Integrated DNA Technologies) used to quantify gene expression by PCR.
DNA from lungs of PbN-infected mice was extracted by illustra tissue & cells genomicPrep Mini spin kit (GE Healthcare). The primers of mouse endogenous gene, GAPDH (Forward: GGCAAATTCAACGGCACAGT and Reverse: AGATGGTGATGGGCTTCCC) and the Plasmodium berghei 18S ribosomal 18S gene (Forward: AAGCATTAAATAAAGCGAATACATCCTTA and 18S Reverse: GGAGATTGGTTTTGACGTTTATGT) were used to determine the parasite load in the lungs by relative quantification, normalized with the mouse GAPDH (ΔCT). The quantitative PCRs were carried out with the Platinum SYBR Green protocol (Invitrogen) on an Applied Biosystems 7500 real-time PCR system.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!