Cells were transduced with LINC00473 shRNA or scrambled shRNA control lentiviruses for 72 hours and then cultured at 5 X 105/well in 6-well plates for additional 96 hours. Cells were then harvested for cell viability via trypan blue exclusion assay and Annexin V-FITC Apoptosis Detection assay (BD Pharmingen). Triplicate assays were set up.
Annexin 5 fitc apoptosis detection assay
Manufactured by BD
Sourced in United Kingdom
The Annexin V-FITC Apoptosis Detection assay is a laboratory product used to detect and quantify apoptosis, a type of programmed cell death. The assay utilizes Annexin V, a protein that binds to phosphatidylserine, a molecule that is translocated to the outer cell membrane during apoptosis. The Annexin V is conjugated with the fluorescent dye FITC, allowing for the detection and measurement of apoptotic cells using flow cytometry or fluorescence microscopy.
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3 protocols using annexin 5 fitc apoptosis detection assay
1
Investigating LINC00473 Knockdown Effects
2
Investigating LINC00473 Knockdown Effects
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Cells were transduced with LINC00473 shRNA or scrambled shRNA control lentiviruses for 72 hours and then cultured at 5 X 105/well in 6-well plates for additional 96 hours. Cells were then harvested for cell viability via trypan blue exclusion assay and Annexin V-FITC Apoptosis Detection assay (BD Pharmingen). Triplicate assays were set up.
3
Apoptosis and Necrosis Assessment of CWP232291
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Cells were seeded in 6-well plates in RPMI 1640 medium containing 10% FBS for 18–24 h. The cells were then exposed to CWP232291 for 72 h, after which apoptosis and necrosis were assessed by flow cytometry using the annexin V-FITC apoptosis detection assay (BD Biosciences, Bedford, MA) in accordance with the manufacturer’s instructions. In this assay, cells positive for annexin V (bottom right quadrant) and those positive for both annexin V and propidium iodide (PI) (top right quadrant) represent the early and late apoptotic populations, respectively, whereas cells positive for PI only (top left quadrant) represent the necrotic population. The apoptosis cell population analysis was carried out using CellQuestPro software (BD Biosciences).
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