Goat anti mouse igg heavy plus light chain h l alexa fluor 488

The IFA was performed as described previously [29 (link)]. Monolayers of MDCK cells were infected at a multiplicity of infection (MOI) of 0.5 with virus. At 24 h post-infection, the cells were fixed with 4% paraformaldehyde and permeabilized with 0.5% Triton X-100. After washing with phosphate-buffered saline (PBS), cells were blocked with 3% bovine serum albumin (BSA) solution. The blocking solution was removed and 10 µg/ml of each mAb was incubated for 1 h at room temperature. Fixed cells were rinsed, and then incubated with a 1:1000 dilution of the goat anti-mouse IgG heavy plus light chain (H + L)–Alexa Fluor 488 (Abcam). Cells were rinsed again, and antibody binding was evaluated using an Image-Pro Plus image system with a GFP imaging cube. Representative images were taken and appropriately labeled by experiment. Isotype antibodies (IgG1 or IgG2a) served as negative controls for the IFA experiments.

The IFA was performed as described previously [29 (link)]. Monolayers of MDCK cells were infected at a multiplicity of infection (MOI) of 0.5 with virus. At 24 h post-infection, the cells were fixed with 4% paraformaldehyde and permeabilized with 0.5% Triton X-100. After washing with phosphate-buffered saline (PBS), cells were blocked with 3% bovine serum albumin (BSA) solution. The blocking solution was removed and 10 µg/ml of each mAb was incubated for 1 h at room temperature. Fixed cells were rinsed, and then incubated with a 1:1000 dilution of the goat anti-mouse IgG heavy plus light chain (H + L)–Alexa Fluor 488 (Abcam). Cells were rinsed again, and antibody binding was evaluated using an Image-Pro Plus image system with a GFP imaging cube. Representative images were taken and appropriately labeled by experiment. Isotype antibodies (IgG1 or IgG2a) served as negative controls for the IFA experiments.