Total proteins were extracted from OCLs formed from OCL precursors derived from these genotypes, and the cell lysates (25 µg/lane) loaded on sodium dodecyl sulfate (SDS) gels using the Bio‐Rad Mini‐gel System (Bio‐Rad Laboratories). The resolved proteins were transferred onto nitrocellulose membranes (TGX‐Membrane; Bio‐Rad) using Trans‐Blot Turbo System Bio‐Rad) and incubated in blocking solution (5% nonfat dry milk in TBST) for 1 h. Membranes were then exposed to primary antibodies overnight at 4°C and incubated with IgG horseradish peroxidase‐conjugated antibody for 1 h. The blots were washed and visualized by an Immobilon Western Chemiluminescent detection system (Thermo Fisher Scientific). Protein expression levels were quantitated by densitometry using ImageJ software (NIH).
Tgx membrane
Manufactured by Bio-Rad
The TGX-Membrane is a laboratory equipment product manufactured by Bio-Rad. It is a high-performance membrane designed for use in various analytical techniques. The core function of the TGX-Membrane is to facilitate efficient protein transfer and separation during electrophoresis procedures.
Automatically generated - may contain errors
2 protocols using tgx membrane
1
Quantitative Western Blot Analysis
2
Western Blot Analysis of Osteoclast Proteins
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