Anti il 18

Manufactured by Proteintech

Sourced in United States

Anti-IL-18 is an antibody product from Proteintech. It is designed to detect and quantify Interleukin-18 (IL-18) protein levels in various sample types. The antibody can be used in immunoassay applications such as ELISA.

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Lab products found in correlation

Anti il 1β, by Proteintech (1 mentions) Dylight 488, by EarthOx (1 mentions) Anti asc, by Cell Signaling Technology (1 mentions) Ix 73, by Olympus (1 mentions) Anti caspase 1, by Proteintech (1 mentions) Rabbit anti nlrp3, by Proteintech (1 mentions) Anti caspase 1, by Cell Signaling Technology (1 mentions) Anti β actin, by Santa Cruz Biotechnology (1 mentions) Anti txnip, by Abcam (1 mentions) Anti nlrp3, by Proteintech (1 mentions) Anti il 1β, by Abcam (1 mentions) Chemidoc xrs system, by Bio-Rad (1 mentions) Anti actin, by Proteintech (1 mentions) Anti nlrp3, by Boster Bio (1 mentions) Anti asc, by Bioss Antibodies (1 mentions) Anti cleaved caspase 1, by Cell Signaling Technology (1 mentions) Anti il 1β, by Bioss Antibodies (1 mentions)

4 protocols using anti il 18

Immunofluorescence Analysis of Inflammasome Proteins

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With regard to the immunofluorescence method, myocardial tissue slices were incubated with the following primary antibodies: Rabbit anti-NLRP3 (1:100, Proteintech, 19771-1-AP, United States), anti-Caspase 1 (1:50, Proteintech, 22915-1-AP, United States), anti-IL-1β (1:200, Proteintech, 26048-1-AP, United States), anti-IL-18 (1:400, Proteintech, 10663-1-AP, China), anti-ASC (1:800, Cell Signaling, #67824, United States).
The slices were then washed and detected with appropriate Fluor dye, DyLight 488 (1:50, EarthOx, E032220-01, United States), as secondary antibodies followed by counterstaining with DAPI in the dark. Afterward, immunofluorescent signaling was observed with a fluorescence microscope (IX73 Olympus). Digital images and data were recorded and analyzed by applying ImageJ (NIH).

Xu L., Zeng Z., Niu C., Liu D., Lin S., Liu X., Szabó G., Lu J., Zheng S, & Zhou P. (2023). Normothermic ex vivo heart perfusion with NLRP3 inflammasome inhibitor Mcc950 treatment improves cardiac function of circulatory death hearts after transplantation. Frontiers in Cardiovascular Medicine, 10, 1126391.

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Immunohistochemical Analysis of Caspase-1 and IL-18

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Tissue sections were dewaxed, dehydrated. Antigen was retrieved in microwave in sodium citrate solution. Sections were then incubated with 3% H₂O₂ for 15 min and blocked with mouse immunoglobulin G blocking reagent (Vector Laboratories, Burlingame, CA) for 1 h. Sections were incubated with anti-caspase-1 (Cell Signaling) or anti-IL-18 (Proteintech) polyclonal antibodies at a dilution of 1:200 for 30 minutes, and then incubated with goat anti-rabbit HRP-labeled antibodies for 30 min. Sections were observed with a light microscope after counterstaining. Control sections were incubated with PBS instead of the specific primary antibodies.

Wang Y., Qin Y., Wang T., Chen Y., Lang X., Zheng J., Gao S., Chen S., Zhong X., Mu Y., Wu X., Zhang F., Zhao W, & Zhong Z. (2018). Pyroptosis induced by enterovirus 71 and coxsackievirus B3 infection affects viral replication and host response. Scientific Reports, 8, 2887.

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Western Blot Analysis of Inflammasome Proteins

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Protein samples extracted from renal tissues or HK-2 cells were used. SDS-PAGE and PVDF membrane transfer were performed as previously described (Wang et al., 2019 (link)). After blocking for 1 h at room temperature, the following primary antibodies were used: anti-GSDMD-N (1:1000, CST, USA), anti-cleaved caspase-1 (1:1000, CST), anti-NLRP3 (1:1000, Proteintech), anti-IL-1β (1:1000, Abcam), anti-IL-18 (1:1000, Proteintech), anti-TXNIP (1:1000, Abcam), and anti-β-actin (1:1000, Santa Cruz, USA). Secondary antibodies were used at a dilution of 1:5000. Signals were detected with a ChemiDoc XRS system (Bio-Rad, CA, USA). ImageJ software (NIH, Bethesda, MD, USA) was used to quantify the protein bands against β-actin.

Li N., Zhao T., Cao Y., Zhang H., Peng L., Wang Y., Zhou X., Wang Q., Li J., Yan M., Dong X., Zhao H, & Li P. (2021). Tangshen Formula Attenuates Diabetic Kidney Injury by Imparting Anti-pyroptotic Effects via the TXNIP-NLRP3-GSDMD Axis. Frontiers in Pharmacology, 11, 623489.

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Western Blot Analysis of Inflammasome Proteins

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Samples were subjected to 12% SDS-PAGE and were then transferred to nitrocellulose filter membranes. Membranes were blocked in 5% skim milk at 25 °C for 1.5 hours and were then incubated with the primary antibodies. Anti-Caspase-1 (1 µg/mL; BOSTER), anti-NLRP3 (1 µg/mL; BOSTER), anti-IL-1β (2 µg/mL; BIOSS, Shanghai, China), anti-IL-18 (0.76 µg/mL; Proteintech), anti-ASC (2 µg/mL; BIOSS, Shanghai, China), anti-PDCD6 (programmed cell death protein 6; 0.734 µg/mL; Proteintech), anti-Cleaved Caspase-1(1 µg/mL;Cell Signaling Technology), and anti-Actin (0.22 µg/mL; Proteintech) antibodies were used in this study. After 4 washes with PBS, membranes were incubated and reacted with horseradish peroxidase-conjugated secondary antibodies. Immunoreactive bands were developed with enhanced chemiluminescence reagents (Amersham, United Kingdom).

Jiang Y., Liu H., Yu H., Zhou Y., Zhang J., Xin W., Li Y., He S., Ma C., Zheng X., Zhang L., Zhao X., Wu B., Jiang C, & Zhu D. (2021). Circular RNA Calm4 Regulates Hypoxia-Induced Pulmonary Arterial Smooth Muscle Cells Pyroptosis via the Circ-Calm4/miR-124-3p/PDCD6 Axis. Arteriosclerosis, Thrombosis, and Vascular Biology, 41(5), 1675-1693.

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