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15n choline chloride

Manufactured by Merck Group

15N-choline chloride is a stable isotope-labeled compound that contains the 15N isotope of nitrogen. It is commonly used as a tracer in various research applications, particularly in the study of choline metabolism and transport.

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2 protocols using 15n choline chloride

1

Isotope Labeling for Lipid Analysis

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99% 13C6-glucose was purchased from Cambridge Isotope Laboratories. 98% 15N-choline chloride was from Sigma. N-palmitoyl-d31-D-erythro-sphingosylphosphorylcholine (2H31-sphingomyelin) and 1,2-dioleoyl-sn-glycero-3-phosphate (sodium salt) (DOPA) were from Avanti Polar Lipids. All other reagents were from Fisher and were used as supplied.
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2

Cholesterol and Phospholipid Synthesis

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Phospholipids and unlabeled cholesterol were from Avanti Polar Lipids. 13C-Methyl iodide, 15N-choline chloride, 2,3,4-13C3-cholesterol, and 25,26,27-13C3-cholesterol were from Sigma. Texas Red 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (TR-DHPE) was from Thermo Fisher Scientific. Four inch 〈100〉 p-type silicon wafers were from Silicon Quest International. Solvents and other chemicals were from Fisher and used as supplied.
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