Goat anti rabbit igg h l horseradish peroxidase hrp secondary antibody

DMEM medium, DMEM red-phenol-free medium, RPMI 1640 medium, RPMI 1640 red-phenol-free medium, fetal bovine serum (FBS), L-glutamine and penicillin-streptomycin were purchased from Gibco BRL (Cergy-Pontoise, France). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), N-acetyl-L-cysteine (NAC), 3-methyladenine (3-MA), anti-β-actin antibody, 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolocarbocyanine iodide (JC-1) and cell death detection enzyme-linked immunosorbent assay^PLUS (ELISA) were obtained from Sigma-Aldrich (Saint-Quentin-Fallavier, France). Beclin-1, Atg5 and LC3 antibodies were acquired from Cell Signaling Technology—Ozyme (Saint-Quentin-en-Yvelines). 2’,7’-dichlorofluorescein diacetate (DCFDA) cellular ROS detection assay kit and goat anti-rabbit IgG H&L horseradish peroxidase (HRP) secondary antibody were purchased from Abcam (Paris, France). LysoTracker, MitoTracker, rabbit anti-mouse IgG-IgM H&L HRP secondary antibody, TO-PRO-3, annexin V-FITC and propidium iodide (PI) were obtained from Invitrogen—Thermo Fisher Scientific (Villebon-sur-Yvette, France). Immobilon Western Chemiluminescent HRP Substrate was acquired from Merck (Lyon, France). Caspase-3/7 green reagent was purchased from Sartorius (Göttingen, Germany).