Venous blood samples were drawn at the catheter laboratory before angiography from ACS patients and from healthy controls during medical examination. Blood biochemistry including total cholesterol (TC), triglyceride (TG), low-density lipoprotein-cholesterol (LDL-C) and high-density lipoprotein-cholesterol (HDL-C) in all participants were performed using the commercially available automated platform (Dimension AR/AVL Clinical Chemistry System, DADE Bchring, Newark, NJ), in the Central Laboratory of the First Affiliated Hospital of Xinjiang Medical University. We also randomly selected some ACS patients and healthy controls to test plasma MIF levels by using enzyme linked immunosorbent assay (ELISA). The randomization of participants selection was computer generated at the Key Laboratory of Cardiovascular Disease Research of Xinjiang Medical University (blocked randomization) and the participants’ allocations were kept in sequentially numbered and sealed envelopes. Plasma MIF levels were measured using Quantikine MIF ELISA kits (R&D Systems, USA) according to manufacturer’s specifications at Xinjiang Key Laboratory of Cardiovascular Disease Research.
Quantikine mif elisa kits
Manufactured by R&D Systems
Sourced in United States
The Quantikine MIF ELISA kits are enzyme-linked immunosorbent assay (ELISA) products designed to quantitatively measure the levels of Macrophage Migration Inhibitory Factor (MIF) in cell culture supernates, serum, plasma, and other biological fluids.
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3 protocols using quantikine mif elisa kits
1
Measuring MIF Levels in ACS Patients
2
Plasma MIF Measurement by ELISA
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Blood samples were drawn by venipuncture into vacationer tubes containing heparin lithium. Plasma was isolated from whole blood by centrifugation and kept in –80°C until experiment in March 2015. Plasma MIF was measured in duplicates using Quantikine MIF ELISA kits (DMF00B, R&D Systems) according to manufacturer's specifications.
3
Measuring Plasma MIF Levels in AMI
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Immediately after admission, venous blood samples were collected into vacutainer tubes containing heparin lithium prior to primary PCI. Within 30 min after collection, samples were centrifuged at 3000 rpm for 10 min at 4 C. Plasma was prepared and stored in aliquots at -80 C until analysis. Repeated freeze-thaw cycles were avoided. MIF level was measured, in duplicates, using Quantikine MIF ELISA kits (DMF00B, R&D Systems) according to manufacturer's specifications. The coefficient of variation for intra-and inter-assay variation was 2.8 ± 1.6% and 5.8 ± 1.3%, respectively. For comparison, we also measured MIF level of healthy people (n = 65) and of patients presenting to the emergency department of the Third Hospital with chest pain but excluded cardiac ischaemia as aetiology (n = 600). All assays were performed by personnel blinded to patient's identity and outcome.
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