Lung tissue immunohistochemistry (IHC) was performed by an independent company (BioTools Co., Ltd; New Taipei City, Taiwan). Briefly, antigen retrieval was carried out by incubating tissues for 20 min in citrate buffer followed by bovine serum albumin (BSA) blocking. Primary antibodies raised against SARS-CoV-2 nucleoprotein (NP; Genetex; product number: GTX135357; 1:200 dilution), ionized calcium-binding adaptor protein-1 (Iba-1; Genetex; 1:100 dilution), Ki-67 (Santa Cruz Biotechnology, Dallas, TX, USA; product number: sc-23900; 1:50 dilution), myeloperoxidase (MPO; Santa Cruz Biotechnology; product number: sc- 365436; 1:50 dilution), Mx1 (Santa Cruz Biotechnology; product number: sc-50509; 1:50 dilution) or anti-CD3 (Ventana Medical Systems, Tucson, AZ, USA; product number: 790-4341) were applied for 32 min using the automated Ventana Benchmark XT platform (Ventana Medical Systems). Labeling was carried out with the Ultraview DAB Detection Kit (Ventana Medical Systems) according to the manufacturer’s protocol. All IHC-stained sections were counterstained with hematoxylin and eosin (H&E) using the Ventana reagent, scanned with Aperio ScanScope CS2 (Leica Biosystems, Buffalo Grove, IL, USA), and analyzed with an Aperio Imagescope v12.3 (Leica Biosystems). Default parameters of the Positive Pixel Count (hue = 0.1; width = 0.5) were used for antigen detection.
Myeloperoxidase mpo
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Myeloperoxidase (MPO) is an enzyme found in the granules of neutrophils and monocytes. It catalyzes the formation of hypochlorous acid from hydrogen peroxide and chloride ions, which plays a role in the microbicidal activity of phagocytes.
Automatically generated - may contain errors
Lab products found in correlation
Ventana benchmark xt platform, by Roche (1 mentions)
Ultraview dab detection kit, by Roche (1 mentions)
Anti cd3, by Roche (1 mentions)
Aperio scanscope cs2, by Leica (1 mentions)
Neutral buffered formalin solution, by Merck Group (1 mentions)
Hrp conjugated secondary antibody, by Santa Cruz Biotechnology (1 mentions)
2 protocols using myeloperoxidase mpo
1
Lung Tissue Immunohistochemistry for SARS-CoV-2
2
Lung Tissue Analysis: Immunohistochemical Profiling
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Lung tissues were harvested from mice post euthanasia and were fixed in 10% neutral buffered formalin solution (Sigma). Then, the formalin-fixed tissues were embedded in paraffin and sectioned into 5 µm thick sections. Later, it was subjected to the standard procedure of deparaffination along with rehydration in continuation with antigen retrieval. Then, subsequent probing by Klf4, Ki67, interleukin 1 beta (IL1β), and myeloperoxidase (MPO) (Santa Cruz Biotechnology) antibodies with corresponding HRP conjugated secondary antibodies (Santa Cruz Biotechnology). 3.3’-Diaminobenzidine (DAB) stating procedure was used for immune-histolochemical development. Later, slides were analyzed under a light microscope (MEIJI, Saitama, Japan) and quantified by Image J software, vs. 1.8.0_111.
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