Rats of SCI group (n = 3) were deeply anesthetized with avertin and perfused transcardially with 0.9% NaCl supplemented, followed by 4% paraformaldehyde on postoperative day 7. Lumbar enlargement tissues were cut into 20 μm cryostat sections (n = 3/each sample) after fixation (4% paraformaldehyde for 24 h in 4°C) and sucrose gradient dehydration. Next, the sections were treated with citric acid to retrieve antigen and then blocked with 10% donkey serum for 30 min at 37°C. The sections were then incubated in the rabbit anti-phospho-STAT3 (Tyr705) (1:200; Cell Signaling Technology, United States), mouse anti-GFAP (1:500; Servicebio, China), mouse anti-Iba-1(1:500; Servicebio, China), and mouse anti-NeuN (1:100; Servicebio, China) overnight at 4°C. After the primary antibody incubation, the sections were incubated with the corresponding secondary antibodies conjugated with CY3 and FITC for 1 h in dark conditions at 37°C. Finally, an Olympus BX60 microscope (Olympus, Japan) was applied to acquire images of the specimens.
Mouse anti neun
Manufactured by Wuhan Servicebio Technology
Sourced in China
Mouse anti-NeuN is a primary antibody that specifically recognizes the neuronal nuclear protein NeuN. NeuN is a widely used marker for identifying mature neurons in the central and peripheral nervous systems.
Automatically generated - may contain errors
Lab products found in correlation
Bx60 microscope, by Olympus (1 mentions)
Anti phospho stat3 tyr705, by Cell Signaling Technology (1 mentions)
Mouse anti iba1, by Wuhan Servicebio Technology (1 mentions)
Confocal laser scanning microscopy, by Nikon (1 mentions)
Mouse anti brdu, by Merck Group (1 mentions)
Rabbit anti cd31, by Wuhan Servicebio Technology (1 mentions)
Rabbit anti nestin, by Santa Cruz Biotechnology (1 mentions)
2 protocols using mouse anti neun
1
Immunohistochemical Analysis of Spinal Cord Injury
2
Histological Analysis of Brain Tissue Post-Cell Transplantation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Two days after cell transplantation, the head of the rats was severed, and the left brain tissue was removed and fixed in 4% paraformaldehyde for 1–3 days. For dehydration, 30% sucrose paraformaldehyde was applied. Paraffin sections, 4-μm thick, were prepared, and morphological and histopathological observations were carried out under a light microscope after HE staining. For double-immunofluorescence labeling, brain tissue was sectioned to 15 μm after dehydration, and these sections were immunostained with monoclonal mouse anti-BrdU (Sigma–Aldrich, USA), rabbit anti-Nestin (Santa Cruz, USA), rabbit anti-CD31 (Servicebio, China), and mouse anti-NeuN (Servicebio, China) antibodies at 4 °C for 20 h. The primary antibodies were visualized using Alexa Fluor 488 chicken anti-mouse IgG and Cy3-conjugated Affinipure goat anti-rabbit IgG. Laser scanning confocal microscopy (Nikon, Japan) was applied to capture images.
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