To determine what degradation products were being released into solution and if UV spectroscopy would be a viable method to rapidly quantify degradation product concentration, analysis of polymer degradation media was performed on the spent degradation media. One sample at each time point was analyzed using a Waters system (Milford, MA) consisting of a 2695 Separations Module with a 2487 dual λ absorbance detector. Empower software was used for data collection and processing. Samples were resolved at room temperature on an Xterra reversed phase RP18 5μm column (4.6 × 150 mm). UV detection of eluted components was performed at λ = 278 nm. Mobile phase consisted of acetonitrile:water (95:5) with ∼1% phosphoric acid, adjusted to pH =2.5 with hydrochloric acid at a flow rate of 1 mL/min. Samples were filtered through 0.22 μm PVDF filters (Fisher) prior to injection.
Pvdf filters
Manufactured by Thermo Fisher Scientific
PVDF filters are a type of laboratory filtration equipment. They are designed to filter liquids and gases, separating particulates or solutes from the sample. PVDF (Polyvinylidene fluoride) is the material used to construct the filter membranes. These filters are versatile and can be used in a variety of laboratory applications.
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2 protocols using pvdf filters
1
UV Spectroscopic Analysis of Polymer Degradation
2
Flexible Tissue Culture Modeling
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Flexible, transparent tissue culture models were prepared by curing Sylgard® 184 silicone elastomer (Dow Corning) around a 2 mm diameter stainless steel rod (McMaster-Carr, 1256T999) in a Plexiglas mold for 24 h at 45°C. To sterilize for cell culture, models were boiled in reverse osmosis water for 30 min. A suitable surface for cell growth was created by coating the inner lumen of the model with 40 μg/mL fibronectin (Sigma, F0985). The models were sealed using PVDF filters (Fisher, 097203) and attached to a LabQuake rotor (Barnstead/Thermolyne) and left to rotate overnight within an incubator set at 37°C and 5% CO2 (Thermo Scientific).
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