Histological procedures were performed as previously described [33 (link)] in the POST-CTRL and BPA groups. Briefly, pieces of ovo-testes were embedded in methacrylate resin (Technovit 7100®, Heraeus Kulzer, Germany). Then, they were sectioned with a microtome (Leica RM2245) and stained with Methylene Blue (Sigma-Aldrich, Munich, Germany)/Azure II (Sigma-Aldrich)/Basic fuchsine (Polysciences, Warrington, PA, USA) [65 (link)]. Being a protandrous hermaphrodite species, gilthead sea bream testes always contain a small quiescent ovarian section, which was also examined.
Azure 2
Manufactured by Merck Group
Sourced in Germany
The Azure II is a high-performance laboratory instrument designed for comprehensive sample analysis. It features advanced optical detection technology and sophisticated data processing capabilities to deliver precise and reliable results. The core function of the Azure II is to enable efficient and accurate analysis of a wide range of sample types.
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Lab products found in correlation
3 protocols using azure 2
1
Histological Examination of Gilthead Sea Bream Testes
2
Ovarian Tissue Histology Preparation
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Ovarian biopsies, obtained during the evaluation of the reproductive stage, were dehydrated in ethanol of gradually increasing concentration (70-96%), and were embedded in methacrylate resin (Technovit 7100®, Heraeus Kulzer, Germany). Using a microtome (Leica RM 2245, Germany), 3 μm sections were cut and stained with Methylene Blue (Sigma, Germany)/Azure II (Sigma, Germany)/Basic Fuchsin (Polysciences, USA) according to the procedure of Bennett et al. (1976) . A compound optical microscope (Nikon, Eclipse 50i, Japan), with a digital camera (Jenoptik progress C12 plus, Germany) mounted on top, was used to examine and photograph the sections.
3
Histological Analysis of Liver Lipids
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Before embedding in methacrylate resin (Technovit 7100®, Heraeus Kulzer, Germany), pieces of liver (n = 10) were dehydrated in gradually increased ethanol solutions (70-96%). Sections of 4 μm were obtained with a microtome (Leica RM2245, Germany) and stained with Methylene Blue (Sigma, Germany)/Azure II (Sigma, Germany)/Basic fuchsine (Polysciences, USA) according to Bennett et al. (1976) . The histological sections were examined under a light microscope (Nikon Eclipse 50i) and microphotographed with a digital camera (Jenoptik progress C12 plus).
The estimation of hepatic lipid area was done according to the methodology of Papadakis (Papadakis et al., 2013) (link). For each liver, 6 microphotographs were obtained at ×40 magnification from different areas of the liver.
The estimation of hepatic lipid area was done according to the methodology of Papadakis (Papadakis et al., 2013) (link). For each liver, 6 microphotographs were obtained at ×40 magnification from different areas of the liver.
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