For glucose quantification in supernatants, a HPX‐87H 300 × 7.8 mm column (Bio Rad) tempered at 25°C was used. The mobile phase consisted of 5 mM sulfuric acid, and the flow rate was set to 0.45 ml/min. Detection was performed with a refractive index detector (Agilent) at 35°C. Samples were filtered and diluted within the calibration range of d (+) glucose (100–2000 mg/l). The injection volume was 20 μl.
Hpx 87h 300 7.8 mm column
Manufactured by Bio-Rad
The HPX‐87H 300 × 7.8 mm column is a laboratory equipment product designed for chromatography applications. It features a 300 mm length and 7.8 mm internal diameter. The core function of this column is to facilitate the separation and analysis of various compounds.
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Lab products found in correlation
2 protocols using hpx 87h 300 7.8 mm column
1
Quantitative Glucose Determination
2
Enzymatic Hydrolysis of Xylan Derivatives
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Solubilized xylan in DES aqueous solutions, precipitated xylan from DES solution and pristine beechwood xylan samples were diluted/solubilized with 50 mmol/L citrate buffer (to reach a final xylan concentration of 100, 50 and 20 g/L) and used as substrates in enzymatic hydrolysis assays (Table 2) using commercial enzymes (CellicTec2, kindly supplied by Novozymes) at an enzyme loading of 4800 UI/g xylan. All assays were conducted in duplicate.
Xylanase activity was measured following the procedure previously described (Bailey et al., 1992) (link) and was found to be 9764 UI/mL (expressed as µmol/mL•min), corresponding to 69 mg of protein/mL quantified by Bradford method (Bradford, 1976) (link). Enzymatic experiments were carried out in an orbital shaker incubator at 50 °C and 150 rpm. The pH was adjusted to 4.8-5 with aqueous NaOH 4 mol/L. Aliquots of the reaction media were withdrawn at desired times and analyzed by HPLC (JASCO), using a BioRad HPX-87H (300 × 7.8 mm) column, at 60 °C with 0.005 M aqueous sulfuric acid as eluent in a flow rate of 0.6 mL/min and a Knauer-IR refractive index detector. The yield of enzymatic saccharification of xylan was determined by the following equation.
Xylanase activity was measured following the procedure previously described (Bailey et al., 1992) (link) and was found to be 9764 UI/mL (expressed as µmol/mL•min), corresponding to 69 mg of protein/mL quantified by Bradford method (Bradford, 1976) (link). Enzymatic experiments were carried out in an orbital shaker incubator at 50 °C and 150 rpm. The pH was adjusted to 4.8-5 with aqueous NaOH 4 mol/L. Aliquots of the reaction media were withdrawn at desired times and analyzed by HPLC (JASCO), using a BioRad HPX-87H (300 × 7.8 mm) column, at 60 °C with 0.005 M aqueous sulfuric acid as eluent in a flow rate of 0.6 mL/min and a Knauer-IR refractive index detector. The yield of enzymatic saccharification of xylan was determined by the following equation.
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