DNA was extracted using the DNeasy plant mini extraction kit (Qiagen, UK). The presence of ELF3-D1 was confirmed by PCR using the TaELF3-D1 primers listed in Table 5 . PCR was performed using the DreamTaq Green PCR kit (Thermo Scientific, UK) on a ProFlex PCR system (Thermo Scientific, UK). PCR reaction conditions were as follows: 3 min at 95°C; 40 cycles of 30 s at 95°C, 30 s at 68°C, 90 s at 72°C; 15 min at 72°C. PCR products were sanger sequenced (Azenta Life Sciences, UK) with sequences mapped to ELF3-D1 (Supplemental Table 10 ) using the Geneious 11.1.1 Read Mapper tool with default settings.
Dreamtaq green pcr kit
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom, United States
The DreamTaq Green PCR kit is a ready-to-use PCR solution that contains DreamTaq DNA Polymerase, dNTPs, MgCl2, and a green dye for direct gel loading. It is designed for routine PCR amplification of DNA fragments.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using dreamtaq green pcr kit
1
Genomic DNA Extraction and ELF3-D1 Identification
2
MMP-9 Gene Amplification Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The amplification of the MMP-9 gene was made using the Thermo Scientific Dream Taq Green PCR kit (Thermo Fisher Scientific, Waltham, MA USA). This kit contains a prepared Master Mix, which is a ready-to use solution containing DreamTaq DNA polymerase, optimized buffer, MgCl2, and dNTPs (deoxynucleotide triphosphates). The master mix is completed with two tracking dyes and a density reagent that allows direct loading of the PCR product onto a gel. The dyes in the master mix do not interfere with the performance of the PCR. It is capable of amplifying genomic DNA.
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