Imaging was performed on a confocal microscope Leica TCS SP5II HCS A with a HC PL APO 20×/0.7 CS (air) objective. DAPI was excited with UV (diode 405 nm/50 mW), Alexa Fluor 488 with an Argon 488 nm laser, and Alexa Fluor 594 with a DPSS (561 nm/20 mW) laser.
Tcs sp5ii hcs a
Manufactured by Leica
Sourced in Germany
The TCS SP5II HCS A is a laser scanning confocal microscope system designed for high-content screening applications. It provides advanced imaging capabilities and flexible configurations to support a wide range of research and analysis needs.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using tcs sp5ii hcs a
1
Confocal Microscopy Imaging Protocol
2
Confocal Imaging of Plant Meristems
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fluorescent lines were observed using an inverted LSM700 confocal microscope with a water-dipping 40x lense. Dissected inflorescence (up to P6, i.e. when the meristem is visible) or NPA pins were removed from the plant and transferred onto solid ½ MS in small round Petri dishes. Cell walls were stained with propidium iodide (10 μg/mL). Just before observation, dissected inflorescences were submerged with sterile distilled water and NPA pins submerged with liquid Arabidopsis medium containing 10μM NPA with or without auxin (NAA at 1μM). Confocal images were analyzed using Fiji (https://fiji.sc/ ). Quantification of fluorescence was carried out as described previously (Larrieu et al., 2014 (link)). Inflorescences used for in situs as well as dissected siliques were observed using a dissecting microscope. Roots were stained with propidium iodide (10 μg/mL) and observed using Leica TCS SP5 II HCS-A confocal microscope with a water-dipping 60x lens.
3
Cell Morphology Analysis of MG-63 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MG-63 cells were seeded
onto the samples in 48-well plates at a density of 2 × 104 cells per well and then cultivated in the medium for 3 and
7 days. At each time point, the samples were fixed with 4% paraformaldehyde
for 10 min and then washed with PBS three times. Before observation
by confocal laser scanning microscopy (Leica TCS SP5 II HCS A, Germany),
the cells were permeabilized with 0.1% Triton X-100 for 5 min, rinsed
with PBS, and blocked with 1% bovine serum albumin solution for 20
min. After that, the cytoskeletons were stained with Alexa Fluor 488
phalloidin for 20 min and nuclei were stained using 4′,6-diamidino-2-phenylindole
(DAPI) for 5 min.
To observe the cell morphologies by SEM, MG-63
cells were cultured onto the samples in 48-well plates at a density
of 2 × 104 cells per well for 3 and 7 days. At each
time point, cell-seeded samples were fixed in 2.5% glutaraldehyde
for 30 min, rinsed with PBS several times, and then dehydrated through
concentration-graded ethanol at 30, 50, 70, 80, 90, and 100% for 15
min each. The dehydrated samples were sputter-coated with platinum
before SEM observation (Quanta 250 FEG, FEI, USA).
onto the samples in 48-well plates at a density of 2 × 104 cells per well and then cultivated in the medium for 3 and
7 days. At each time point, the samples were fixed with 4% paraformaldehyde
for 10 min and then washed with PBS three times. Before observation
by confocal laser scanning microscopy (Leica TCS SP5 II HCS A, Germany),
the cells were permeabilized with 0.1% Triton X-100 for 5 min, rinsed
with PBS, and blocked with 1% bovine serum albumin solution for 20
min. After that, the cytoskeletons were stained with Alexa Fluor 488
phalloidin for 20 min and nuclei were stained using 4′,6-diamidino-2-phenylindole
(DAPI) for 5 min.
To observe the cell morphologies by SEM, MG-63
cells were cultured onto the samples in 48-well plates at a density
of 2 × 104 cells per well for 3 and 7 days. At each
time point, cell-seeded samples were fixed in 2.5% glutaraldehyde
for 30 min, rinsed with PBS several times, and then dehydrated through
concentration-graded ethanol at 30, 50, 70, 80, 90, and 100% for 15
min each. The dehydrated samples were sputter-coated with platinum
before SEM observation (Quanta 250 FEG, FEI, USA).
4
Imaging NMDA-induced Actin Dynamics
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cultured hippocampal neurons were transfected at 11 DIV with mKATE-α-actinin construct (Evrogen) and monitored at 12 DIV with a high-content confocal microscope (TCS SP5 II, HCSA, Leica) using the 63× objective. Neurons were transferred to HBSS/Ca++ medium and monitored for 10 min before NMDA treatment. The same volume of HBSS/Ca++ medium containing 40 µM NMDA was added to cultures and incubated for 5 min before restarting imaging. The same neuron was imaged another 20 min. Imaging was done at +37°C in 5% CO2.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!