Sodium citrate based er1 buffer

Manufactured by Leica Biosystems

Sodium citrate-based ER1 buffer is a laboratory reagent used for antigen retrieval in immunohistochemistry (IHC) and immunofluorescence (IF) procedures. It is designed to help unmask target antigens in fixed tissue samples, facilitating the binding of specific antibodies during the staining process.

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Lab products found in correlation

Anti iba1 antibody, by Fujifilm (2 mentions) Bond rx, by Leica Biosystems (1 mentions) Bond rx automated system, by Leica Biosystems (1 mentions)

2 protocols using sodium citrate based er1 buffer

Histological Analysis of Hamster Lung Tissue

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Formalin-fixed and paraffin-embedded tissue sections were stained with hematoxylin and eosin (H&E) or anti-Iba-1 antibody (Wako; 019–19,741 Richmond, VA; at a dilution of 1:2000). Morphometric analyses were performed on affected lung tissues using Image J software (NIH, USA) [22 (link)]. A minimum of three fields of view were obtained from each animal (n = 6 animals; 3 male and 3 female hamsters). Heat-induced epitope retrieval was conducted by heating slides to 95 °C for 20 min in sodium citrate–based ER1 buffer (Leica Biosystems, Richmond, IL) before immunostaining. Immunostaining was performed using the Bond RX automated system (Leica Biosystems, Richmond, IL). Positive immunostaining was visualized using diaminobenzidine tetrahydrochloride (DAB) and slides were counterstained with hematoxylin.

Ruiz-Bedoya C.A., Mota F., Ordonez A.A., Foss C.A., Singh A.K., Praharaj M., Mahmud F.J., Ghayoor A., Flavahan K., De Jesus P., Bahr M., Dhakal S., Zhou R., Solis C.V., Mulka K.R., Bishai W.R., Pekosz A., Mankowski J.L., Villano J., Klein S.L, & Jain S.K. (2021). 124I-Iodo-DPA-713 Positron Emission Tomography in a Hamster Model of SARS-CoV-2 Infection. Molecular Imaging and Biology, 24(1), 135-143.

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TREM2 and IBA1 Expression in SIV-Infected Brain

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TREM2 ISH was combined with IBA1 immunostaining performed on frontal cortex sections from an SIV infected animal. TREM2 ISH was performed as described above. The first heat-induced epitope retrieval was conducted by heating slides to 95°C in EDTA-based ER2 buffer for 15 minutes (Leica Biosystems, Richmond, IL). Slides were then stained with anti-IBA1 antibody (cat. 01919741, Wako, Richmond, VA) at a dilution of 1:500. IHC was performed using the Bond RX automated system (Leica Biosystems, Richmond, IL). A second heat-induced epitope retrieval was conducted by heating slides to 95°C for 20 minutes in sodium citrate-based ER1 buffer (Leica Biosystems, Richmond, IL). Positive immunostaining was visualized using DAB, and slides were counterstained with hematoxylin.

Knight A.C., Brill S.A., Solis C.V., Richardson M.R., McCarron M.E., Queen S.E., Bailey C.C, & Mankowski J.L. (2020). Differential Regulation of TREM2 and CSF1R in CNS Macrophages in an SIV/Macaque Model of HIV CNS Disease. Journal of neurovirology, 26(4), 511-519.

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