30 kda cut off centrifugal concentrator

Manufactured by Merck Group

The 30 kDa cut-off centrifugal concentrator is a laboratory equipment used for concentrating and desalting macromolecular solutions. It employs a semi-permeable membrane with a molecular weight cut-off of 30 kDa to selectively retain and concentrate target molecules while allowing smaller components to pass through.

Automatically generated - may contain errors

Lab products found in correlation

Opti mem, by Thermo Fisher Scientific (4 mentions) 293 free transfection reagent, by Merck Group (2 mentions) Anti flag resin, by GenScript (2 mentions) S protein agarose, by Merck Group (2 mentions) Superose 6 increase 10 300 gl column, by Cytiva (1 mentions)

3 protocols using 30 kda cut off centrifugal concentrator

Purifying RDH Complex for Cryo-EM

Check if the same lab product or an alternative is used in the 5 most similar protocols

To purify the RDH complex for cryo-EM, size exclusion chromatography was performed. The membrane protein extract was pre-equilibrated in Buffer 3 in order to reduce detergent concentration and loaded in a Superose 6 Increase 10/300 GL column (Cytiva) at a flow rate of 0.5 mL/min on an ÄKTA Go FPLC system (Cytiva). Fractions containing the RDH complex were pooled, concentrated to 1 mg/mL using a 30 kDa cut-off centrifugal concentrator (Millipore), and their purity analysed by SDS-PAGE.

Cimmino L., Duarte A.G., Ni D., Ekundayo B.E., Pereira I.A., Stahlberg H., Holliger C, & Maillard J. (2023). Structure of a membrane-bound menaquinol:organohalide oxidoreductase. Nature Communications, 14, 7038.

+ Open protocol

+ Expand

Production and Purification of Soluble NiV and HeV F

Check if the same lab product or an alternative is used in the 5 most similar protocols

Soluble NiV F and HeV F were produced by transient transfection of FreeStyle 293F cells at a density of 1 × 10⁶ cells/mL with the corresponding plasmid using 293-Free transfection reagent (Millipore) and Opti-MEM (Thermo-Fisher) according to the manufacturer’s protocol. After 5 days in a humidified shaking incubator and maintained at 37°C and 8% CO₂, the cell supernatant was harvested and clarified of cell debris by centrifugation. Subsequent affinity purification was carried out using an anti-FLAG resin (Genscript) and elution with 1 mg/mL FLAG peptide dissolved in Tris buffer pH 8.0, 150mM NaCl or with S-protein agarose (Millipore Sigma, Novagen) and elution with 0.2 M citric acid pH 2.0 followed by immediate neutralization with 1.0 M Tris pH 9.5. The eluted fraction was buffer-exchanged into 50 mM Tris buffer pH 8.0, 150 mM NaCl using a 30 kDa cutoff centrifugal concentrator (Millipore).

Dang H.V., Chan Y.P., Park Y.J., Snijder J., Da Silva S.C., Vu B., Yan L., Feng Y.R., Rockx B., Geisbert T.W., Mire C.E., Broder C.C, & Veesler D. (2019). An antibody against the F glycoprotein inhibits Nipah and Hendra virus infections. Nature Structural & Molecular Biology, 26(10), 980-987.

+ Open protocol

+ Expand

Soluble NiV and HeV Fusion Protein Production

Check if the same lab product or an alternative is used in the 5 most similar protocols

Soluble NiV F and HeV F were produced by transient transfection of FreeStyle 293F cells at a density of 1 × 10⁶ cells ml⁻¹ with the corresponding plasmid using 293-Free transfection reagent (Millipore) and Opti-MEM (Thermo-Fisher) according to the manufacturer’s protocol. After five days in a humidified shaking incubator, maintained at 37 °C and 8% CO₂, the cell supernatant was harvested and clarified of cell debris by centrifugation. Subsequent affinity purification was carried out using an anti-FLAG resin (Genscript) and elution with 1 mg ml⁻¹ FLAG peptide dissolved in Tris buffer pH 8.0, 150 mM NaCl or with S-protein agarose (Millipore Sigma, Novagen) and elution with 0.2 M citric acid pH 2.0 followed by immediate neutralization with 1.0 M Tris pH 9.5. The eluted fraction was buffer-exchanged into 50 mM Tris buffer pH 8.0, 150 mM NaCl using a 30 kDa cutoff centrifugal concentrator (Millipore).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!