Bio plex human group 2 cytokine 21 plex panel

Manufactured by Bio-Rad

Sourced in United States

The Bio-Plex Human Group II Cytokine 21-Plex Panel is a multi-analyte detection system that simultaneously measures the concentration of 21 different human cytokines in a single sample. The panel includes a selection of Group II cytokines and is designed for use with the Bio-Plex suspension array system.

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Lab products found in correlation

Bio plex human cytokine 27 plex panel, by Bio-Rad (1 mentions)

3 protocols using bio plex human group 2 cytokine 21 plex panel

Cytokine Profiling of DB-ASC Secretome

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The conditioned medium of DB-ASCs (from untreated or treated PTX cells) was collected, and the cytokine content was evaluated using “multiplex bead-based xMAP technology” (Bio-Plex Human Cytokine 27-Plex Panel and Bio-Plex Human Group II Cytokine 21-Plex Panel, Bio-Rad Laboratories).

Coccè V., Brini A., Giannì A.B., Sordi V., Berenzi A., Alessandri G., Tremolada C., Versari S., Bosetto A, & Pessina A. (2018). A Nonenzymatic and Automated Closed-Cycle Process for the Isolation of Mesenchymal Stromal Cells in Drug Delivery Applications. Stem Cells International, 2018, 4098140.

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Multiplex Cytokine Profile Analysis

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The CM from GinPa-MSCs were analysed for their cytokines/chemokines content by using multiplex bead-based assays on xMAP technology (Bio-Plex Human Cytokine 27-Plex Panel; Bio-Plex Human Group II Cytokine 21-Plex Panel; Biorad Laboratories). As the assay is forecasted to analyse 48 different cytokines, in our study we considered only the cytokines that were found in the conditioned media at levels >of 1,500 pg/ml.

Coccè V., Farronato D., Brini A.T., Masia C., Giannì A.B., Piovani G., Sisto F., Alessandri G., Angiero F, & Pessina A. (2017). Drug Loaded Gingival Mesenchymal Stromal Cells (GinPa-MSCs) Inhibit In Vitro Proliferation of Oral Squamous Cell Carcinoma. Scientific Reports, 7, 9376.

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Quantifying MSC Secretome Cytokines

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The conditioned media of standardized cultures of MSCs (1.2 × 10⁴ cells/cm² at 6 days of culture) were analyzed for cytokine content. The qualitative/quantitative analysis was performed by using “multiplex bead-based xMAP technology” (Bio-Plex Human Cytokine 27-Plex Panel, Bio-Plex Human Group II Cytokine 21-Plex Panel, Bio-Rad Laboratories (Hercules, CA, USA)).

Coccè V., La Monica S., Bonelli M., Alessandri G., Alfieri R., Lagrasta C.A., Madeddu D., Frati C., Flammini L., Lisini D., Marcianti A., Parati E., Paino F., Giannì A., Farronato G., Falco A., Spaggiari L., Petrella F, & Pessina A. (2021). Inhibition of Human Malignant Pleural Mesothelioma Growth by Mesenchymal Stromal Cells. Cells, 10(6), 1427.

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