Surface marker analysis by FACS was carried out as described previously [16 (link)]. In brief, hMSC and ES cell lines (RD-ES, SK-N-MC and EWS-GFP) were harvested, centrifugated and incubated at 4°C for 1 h with fluorochrome conjugated antibodies APC Mouse anti-human CD13 (BD Pharmingen, 557454), APC Mouse anti-human CD44 (BD Pharmingen, 560532), APC Mouse anti-human CD73 (BD Pharmingen, 560847), APC Mouse anti-human CD90 (BD Pharmingen, 559869) and APC Mouse anti-human CD105 (BD Pharmingen, 562408). Negative control cells were stained with APC mouse IgG1, k isotype control, Clone MOPC-21 (BD Pharmingen, 555751). CD99 expression was assayed incubating cells with CD99 primary antibody (Signet antibodies, SIG-3620). FACS data were analyzed using FlowJo software version 7.6 (Tree Star Inc., Ashland, OR, USA)
Apc mouse anti human cd13
Manufactured by BD
Sourced in United States
The APC Mouse anti-human CD13 is a flow cytometry antibody used to identify and enumerate cells expressing the CD13 antigen. CD13 is a cell surface metalloprotease enzyme expressed on a variety of cell types, including myeloid cells, endothelial cells, and epithelial cells. This antibody can be used to detect CD13-positive cells in human samples.
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Lab products found in correlation
Apc mouse antihuman cd73, by BD (1 mentions)
Apc mouse igg1, by BD (1 mentions)
Clone mopc 21, by BD (1 mentions)
Apc mouse anti human cd105, by BD (1 mentions)
Apc mouse anti human cd44, by BD (1 mentions)
Apc mouse anti human cd90, by BD (1 mentions)
Apc mouse anti human cd34, by BD (1 mentions)
Pe mouse anti human cd14, by BD (1 mentions)
Pe mouse anti human cd45, by BD (1 mentions)
Fitc mouse anti human hla abc, by BD (1 mentions)
Fitc mouse anti human cd31, by BD (1 mentions)
Pe mouse anti human cd29, by Thermo Fisher Scientific (1 mentions)
2 protocols using apc mouse anti human cd13
1
Surface Marker Analysis of Mesenchymal and Cancer Cells
2
Immunophenotyping of Mesenchymal Stem Cells
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The immunophenotype characterization of the MSCs was conducted using a previously described protocol [1 (link)]. Conjugated monoclonal antibodies against PE-Cy™5 mouse anti-human CD90, PE-Cy™7 mouse anti-human CD73, APC mouse anti-human CD13, FITC mouse anti-human HLA-ABC, APC mouse anti-human CD34, FITC mouse anti-human CD31, PE mouse anti-human CD45, PE mouse anti-human CD14 (BD Biosciences, San Diego, CA, USA), eFluor™450 mouse anti-human CD105, PE mouse anti-human CD29 (eBioscience, San Diego, CA, USA), PE/Cyanine7 mouse anti-human HLA-DR and PE/Cyanine7 mouse anti-human CD10 (BioLegend, San Diego, CA, USA) were used.
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