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Anti aggrecan and anti collagen antibody

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Anti-Aggrecan and anti-collagen antibodies are laboratory reagents used for the detection and quantification of aggrecan and collagen proteins in various biological samples. These antibodies are designed to specifically bind to their target proteins, enabling researchers to study their expression, distribution, and interactions in different experimental settings.

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Lab products found in correlation

Decalcifying solution, by Avantor (2 mentions)

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Aggrecan (93 citations) Anti-Aggrecan (19 citations) Anti-aggrecan antibody (5 citations)

2 protocols using anti aggrecan and anti collagen antibody

1

Histochemical Analysis of Articular Cartilage

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After euthanasia, the joints were excised, trimmed and fixed in 4% paraformaldehyde (PFA) for 48 hr and transferred to a 70% ethanol solution. Joints were partially decalcified for about 4 hr using a rapid decalcifying formic acid/hydrochloric acid mixture (Decalcifying Solution, VWR). The joint area was cut in cross‐section with a razor blade and embedded in paraffin wax. Sections (5 µm) of the cross section were stained with Safranin‐O and imaged using bright‐field microscopy. Heat‐induced epitope retrieval was used prior to applying immunohistochemical stains for detecting aggrecan and collagen. Anti‐Aggrecan and anti‐collagen antibody (Abcam) was used in a 1:200 dilution in Tris‐buffered saline and with an HRP conjugate for detection.
Shah N.J., Geiger B.C., Quadir M.A., Hyder N., Krishnan Y., Grodzinsky A.J, & Hammond P.T. (2016). Synthetic nanoscale electrostatic particles as growth factor carriers for cartilage repair. Bioengineering & Translational Medicine, 1(3), 347-356.
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2

Histological Analysis of Articular Cartilage

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After euthanasia the joints were excised, trimmed and fixed in 4% paraformaldehyde (PFA) for 48 hours and transferred to a 70% ethanol solution. Joints were partially decalcified for about 4 hours using a rapid decalcifying formic acid/hydrochloric acid mixture (Decalcifying Solution, VWR). The joint area was cut in cross-section with a razor blade and embedded in paraffin wax. Sections (5 μm) of the cross section were stained with Safranin-O and imaged using bright-field microscopy. Heat-induced epitope retrieval was used prior to applying immunohistochemical stains for detecting aggrecan and collagen. Anti-Aggrecan and anti-collagen antibody (Abcam) was used in a 1:200 dilution in Tris-Buffered Saline and with an HRP conjugate for detection.
Shah N.J., Geiger B.C., Quadir M.A., Hyder N., Krishnan Y., Grodzinsky A.J, & Hammond P.T. (2016). Synthetic nanoscale electrostatic particles as growth factor carriers for cartilage repair. Bioengineering & Translational Medicine, 1(3), 347-356.
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