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3 protocols using synergic acid

1

Phytochemical Analysis of Plant Extracts

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Milli-Q water (Millipore, Bedford, MA) was used in all experiments. 2,2-Diphenyl-1-picrylhydrazyl hydrate (DPPH), catechin (98%), Folin–Ciocalteu reagent (2N), chlorogenic acid (95%), caffeic acid (98%), ferulic acid (99%), gallic acid (98%), protocatechuic acid (95%), synergic acid (95%), synaptic acid (95%), t -cinnamic acid (95%), and rutin (94%) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Hesperidin (98%), naringenin (98%), narirutin (98%), apigenin (98%), and luteolin (98%) were purchased from Cayman Chemical Company (Ann Arbor, MI, USA). HPLC-grade methanol and acetic acid were acquired from Sigma-Aldrich (St. Louis, MO, USA) and used in the mobile phase. All other chemicals were of analytical grade.
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2

Cholesterol and Biochemical Analysis Protocol

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Pure white crystalline cholesterol powder and saline solutions were purchased from SIGMA Chemical Co., (USA). Casein, cellulose, choline chloride powder, and DL-methionine powder, were obtained from Morgan Co. Cairo, Egypt. Chemical kits used in this study (TC, TG, HDL-c, ALT, AST, ALP, urea, creatinine, serum protein) were obtained from Al-Gomhoria Company for Drugs, Chemical and Medical Instruments, Cairo, Egypt. Folin-Ciocalteu reagent and standard substances including gallic acid, caffeic acid, oleuropein, protocatechuic acid and pcoumaric acid were purchased from Sigma Chemical Company (St. Louis, USA), synergic acid, ferulic acid, rutin and Quercetin from SIGMA Chemical Co., (USA). All reagents and standards were prepared using Milli-Q deionized water (Millipore, Bedford, USA). All other chemicals and reagents were of analytical reagent grade and purchased from Al-Ghomhoria Company, Egypt.
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3

Assay for Salivary α-Amylase and Glucosidase

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Human salivary α-amylase (A0521), lyophilised α-glucosidase from Saccharomyces cerevisiae, EC 232-604-7 (G5003), 4-nitrophenyl α-D-glucopyranoside (pNPG-N1377) and acarbose (A8980) were obtained from Sigma-Aldrich, UK. Cyanidin (79457), delphinidin (43725), delphinidin-3-galactoside (04301), protocatechuic acid (03930590), vanillic acid (94770), protocatechuic aldehyde (D108405), ferulic acid (PHR1791), 4hydroxybenzaldehyde (144088), chlorogenic acid (C3878), gallic acid (G7384), synergic acid (S6881), resveratrol (R5010) were purchased from Sigma-Aldrich, UK. Malvidin (80083), malvidin-3-galactoside (80600), Cyanidin-3-galactoside (89463), Cyanidin-3-arabinoside (89614), petunidin-3-glucoside (89755), pelargonidin-3-glucoside (89753), pelargonidin (80084) and peonidin (80085) were purchased from PhytoLab, Germany. Cyanidin-3glucoside (0915S), delphinidin-3-glucoside (0938S) and malvidin-3-glucoside (0911S), were from Extrasynthese, France.
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