Antibodies against p62 (#610832) were purchased from BD Pharmingen. Antibodies against GAPDH (#5174), LKB1 (#3050S), mTOR (#2972), p-mTOR (S2448; #2971S), AMPKα (#2603), p-AMPK (T172; #2535), ACC (#3676), p-ACC (S79; #3661), ULK1 (#8054) and p-ULK1 (S555; #5869) were purchased from Cell Signaling. Polyclonal antibodies against OGT (#SAB2101676) and OGA (#SAB4200311) were purchased from Sigma-Aldrich. The monoclonal antibody against O-GlcNAc (RL2; #MA1072) was purchased from Thermo Fisher Scientific. Antibodies against LC3 (#NB100-2220SS) were purchased from Novus Biological. The anti-GFP monoclonal antibody (#SC9996) was purchased from Santa Cruz Biotechnology. DON, TG and bafilomycin A1 (Baf A1) were purchased from Sigma-Aldrich. The pcDNA6.2-myc construct containing AMPKα, OGT, OGA or null control and the pLKO shRNA construct containing OGT, OGA, AMPKα or negative control were purchased from Addgene.
Jin L., Yuan F., Dai G., Yao Q., Xiang H., Wang L., Xue B., Shan Y, & Liu X. (2020). Blockage of O-linked GlcNAcylation induces AMPK-dependent autophagy in bladder cancer cells. Cellular & Molecular Biology Letters, 25, 17.
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