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Shgli1

Manufactured by Genechem
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Sourced in China

ShGli1 is a laboratory equipment designed for the analysis and detection of specific genetic sequences. It serves as a tool for researchers and scientists working in the field of molecular biology and genetics.

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Lab products found in correlation

Sh nc, by Genechem (1 mentions) Rnai lentivirus particles, by Genechem (1 mentions) Sh con, by Genechem (1 mentions)

2 protocols using shgli1

1

Lentivirus-mediated Knockdown of β2-AR and Gli1

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Lentivirus vectors containing the small hairpin RNA (shRNA) against β2‐AR (shβ2‐AR) (NM_000024) or Gli1 (shGli1) (NM_005269) and the small hairpin negative control (shNC) were constructed and generated by GeneChem Company (Shanghai, China). Lentiviruses were resuspended in DMEM/F12 or RPMI‐1640 and were added dropwise on cells in six‐well plates in the presence of medium without penicillin‐streptomycin. The selection of lentivirus‐infected cells was achieved with puromycin at a final concentration of 5 μg/mL. β2‐AR and Gli gene expression levels in infected cells were validated by Western blot analysis and real‐time polymerase chain reaction (RT‐PCR). Transfection of a control shRNA at the same concentration served as a control.
Zhang M., Wang Q., Sun X., Yin Q., Chen J., Xu L, & Xu C. (2020). β2‐adrenergic receptor signaling drives prostate cancer progression by targeting the Sonic hedgehog‐Gli1 signaling activation. The Prostate, 80(15), 1328-1340.
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2

Lentiviral Knockdown of WW45 and Gli1

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RNAi lenti-virus particles (sh-con, sh-WW45 and sh-Gli1) were from GeneChem (China). Cells were infected with the indicated lenti-virus particles of the same MOI for 24 hours and then stable knock-down cells were selected with the medium containing puromycin for at least a week.
Li X., Zhou X., Fan Y., Zhang Y., Zu L., Yao F, & Zhou Q. (2016). WW45, a Gli1 binding protein, negatively regulated Hedgehog signaling in lung cancer. Oncotarget, 7(42), 68966-68975.
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