Deionized water was used in all capillary solutions for the equilibration experiments. The polymeric reservoir solutes included PEG 4000 and 8000 (Hampton Research, Aliso Viejo, CA, USA), and PEG 400 (Fluka Chemical, Milwaukee, WI, USA). The PEG reservoir solution concentrations were kept at 15%(w/v) except for PEG 400 which was 15%(v/v). All salt reservoir solutions were kept at 10%(w/v) concentration. The salt precipitants include sodium malonate and lithium sulfate (MP Biomedicals, Irvin, CA, USA), sodium chloride (Sigma–Aldrich, St Louis, MO, USA), sodium potassium tartrate, ammonium phosphate and ammonium sulfate (Fischer Scientific, Pittsburg, PA, USA).
Sodium potassium tartrate
Manufactured by Thermo Fisher Scientific
Sourced in Belgium
Sodium potassium tartrate is a chemical compound that is commonly used as a laboratory reagent. It is a white, crystalline solid that is soluble in water and has a variety of applications in scientific research and analysis.
Automatically generated - may contain errors
Lab products found in correlation
Sodium hydroxide (naoh), by Thermo Fisher Scientific (5 mentions)
Potassium chloride (kcl), by Thermo Fisher Scientific (4 mentions)
Sodium chloride (nacl), by Avantor (4 mentions)
Kh2po4, by Avantor (4 mentions)
Nahco3, by Avantor (4 mentions)
Total starch kit, by Megazyme (4 mentions)
Mgcl2 h2o 6, by Thermo Fisher Scientific (4 mentions)
Sodium chloride (nacl), by Merck Group (1 mentions)
Ammonium sulfate, by Thermo Fisher Scientific (1 mentions)
Trisodium citrate, by Thermo Fisher Scientific (1 mentions)
Epoch microplate spectrophotometer, by Agilent Technologies (1 mentions)
Glass microfiber filter, by Avantor (1 mentions)
Sodium carbonate, by Thermo Fisher Scientific (1 mentions)
Citric acid, by Thermo Fisher Scientific (1 mentions)
Succinic acid, by Thermo Fisher Scientific (1 mentions)
Copper sulfate, by Thermo Fisher Scientific (1 mentions)
O phosphoric acid, by Thermo Fisher Scientific (1 mentions)
Bovine serum albumin fraction 5, by HiMedia (1 mentions)
Whatman no 1 filter paper, by Cytiva (1 mentions)
Whatman, by Cytiva (1 mentions)
6 protocols using sodium potassium tartrate
1
Protein Crystallization Reservoir Preparation
2
Comprehensive Protein Quantification Protocol
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Bromocresol green, citric acid, Coomassie brilliant blue (CBB) (G-250), copper sulfate, Folin-Ciocalteau (FC) reagent, O-phosphoric acid, sodium carbonate, sodium hydroxide, sodium potassium tartrate, succinic acid, and tri-sodium citrate were purchased from Thermo Fisher Scientific India Pvt. Ltd., India. Bicinchoninic acid and tetrabromophenol blue were purchased from Carbosynth Limited, Compton, Berkshire, UK. Sodium bicarbonate was purchased from NICE Chemicals Pvt. Ltd., India. Ethanol (>99%) was purchased from Changshu Jangyuan Chemical, China. Bovine serum albumin (BSA) – Fraction V, purchased from Himedia Laboratories Pvt. Ltd., India, was used as standard protein throughout the research. Reagent grade triple deionized distilled water was purchased from Marech Pvt. Ltd., Lalitpur, Nepal. Whatman No. 1 filter paper was purchased from Whatman International Ltd, Maidstone, England, and glass microfiber filter was purchased from VWR International, Pennsylvania, USA. Epoch™ microplate spectrophotometer (Biotek instruments, Inc., USA) was used for spectrophotometric determination of proteins in serum and urine samples.
3
Green Lentil Starch Quantification
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Green lentils (Dupuy type) from Canada (harvested in August 2019) were donated by Casibeans (Melsele, Belgium). After sorting, the raw seeds (<10% moisture) were stored at −40 °C. Storage below the glass transition temperature prevents biochemical changes (such as the development of the hard-to-cook phenomenon), ensuring stability of the seeds until usage [38 (link),39 (link)]. The Total Starch Kit was obtained from Megazyme (Bray, Ireland). KCl, MgCl2(H2O)6, NaOH, and sodium potassium tartrate were purchased from Acros Organics (Geel, Belgium). NaHCO3, NaCl, and KH2PO4 were obtained from VWR (Leuven, Belgium). All other chemical reagents and enzymes were purchased from Merck KgaA (Darmstadt, Germany).
4
Enzymatic Digestibility of Plant-Based Proteins
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Kabuli chickpeas (CP) (Cicer arietinum, Argentina), dry black turtle beans (BB) (Phaseolus vulgaris, Canada), and dry green peas (PE) (Pisum sativum, Europe) were harvested in 2018/2019 and obtained from Greenyard Prepared (Bree, Belgium) and Casibeans© (Melsele, Belgium) immediately after harvest. Pulse seed material was used in two ways in this work: (i) to be studied as whole seed material (WSM); and (ii) to generate individual cotyledon cells (ICC). Plant material was sorted, cleaned from defect seeds and foreign material, and kept at −40 °C until further use [31 (link)]. Storage conditions were chosen to ensure storage stability of the raw pulse seeds in their dried, low moisture state according to Kyomugasho et al. [36 (link)]. Pepsin (P7012), pancreatin (P1750), trypsin (T2011), chymotrypsin (C4129), and most other chemical reagents were purchased from Sigma Aldrich (Brussels, Belgium), except for KCl, MgCl2(H2O)6, NaOH, sodium potassium tartrate (Acros Organics, Geel, Belgium) NaHCO3, NaCl, KH2PO4 (VWR, Leuven, Belgium), and the Total Starch Kit (Megazyme, Bray, Ireland).
5
Characterizing Lentil Starch Properties
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Raw Dupuy-type green lentils (L. culinaris) grown and harvested in Canada in August 2019, were donated by Casibeans (Melsele, Belgium). The material (<10% moisture) was sorted and foreign material removed, after which seeds were stored below their glass transition temperature (-40°C), ensuring stability until usage (Kyomugasho, Kamau, Aravindakshan, & Hendrickx, 2021) .
Chemical reagents and enzymes were purchased from Sigma Aldrich (Belgium), except for the Total Starch Kit (Megazyme, Bray, Ireland), KCl, MgCl2(H2O)6, NaOH, sodium potassium tartrate (Acros Organics, Geel, Belgium), NaHCO3, NaCl, and KH2PO4 (VWR, Leuven, Belgium).
Chemical reagents and enzymes were purchased from Sigma Aldrich (Belgium), except for the Total Starch Kit (Megazyme, Bray, Ireland), KCl, MgCl2(H2O)6, NaOH, sodium potassium tartrate (Acros Organics, Geel, Belgium), NaHCO3, NaCl, and KH2PO4 (VWR, Leuven, Belgium).
6
Harvesting and Stabilization of Green Lentils
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Green (Dupuy) lentils (L. culinaris) were grown and harvested in Canada (August 2019) and donated by Casibeans (Melsele, Belgium). The material (<10% moisture) was sorted and cleaned from foreign material, followed by storage below the glass transition temperature to ensure stability (-40 °C) . Chemical reagents and enzymes were purchased from Merck KGaA (Germany), except for the Total Starch Kit (Megazyme, Bray, Ireland), KCl, MgCl2(H2O)6, NaOH, sodium potassium tartrate (Acros Organics, Geel, Belgium), NaHCO3, NaCl, KH2PO4 (VWR, Leuven, Belgium).
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