Tris, octylphenoxypolyethoxyethanol (NP-40), urea, sulfourea, 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS), sodium dodecyl sulphate (SDS), acrylamide, N,N′-methylenebisacrylamide, ammonium persulfate, N,N,N′,N′-tetramethylethylene diamine, CBB G-250 and trichloroacetic acid were obtained from Amresco (Solon, OH, USA); phenylmethylsulfonyl fluoride was obtained from Sigma (St. Louis, MO, USA); immobilized pH gradient strips (IPG strips, 17 cm, pH 4–7) and iodoacetamide were obtained from Bio-Rad Laboratories (Hercules, CA, USA); acetone, glycerol, phosphoric acid, carbinol and alcohol (analytical reagents) were obtained from manufacturers in China. All water used in this experiment was Milli-Q hyperpure water (Millipore, Billerica, MA, USA).
Cbb g 250
Manufactured by Avantor
Sourced in United States
CBB G-250 is a dye used for protein quantification. It binds to basic and aromatic amino acid residues in proteins, resulting in a color change that can be measured spectrophotometrically.
Automatically generated - may contain errors
Lab products found in correlation
Iodoacetamide, by Bio-Rad (1 mentions)
Glycerol, by Bio-Rad (1 mentions)
Acrylamide, by Avantor (1 mentions)
Ammonium persulfate, by Avantor (1 mentions)
Acetone, by Bio-Rad (1 mentions)
Sodium dodecyl sulphate (sds), by Avantor (1 mentions)
Trichloroacetic acid, by Avantor (1 mentions)
N n methylenebisacrylamide, by Avantor (1 mentions)
N n n n tetramethylethylene diamine, by Avantor (1 mentions)
Np 40, by Avantor (1 mentions)
Phenylmethylsulfonyl fluoride, by Merck Group (1 mentions)
Readystriptm ipg strips, by Bio-Rad (1 mentions)
Precision plus proteintm unstained standard, by Bio-Rad (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Chicken egg lysozyme, by Merck Group (1 mentions)
Staurosporine, by Merck Group (1 mentions)
Bovine carbonic anhydrase, by Merck Group (1 mentions)
2 protocols using cbb g 250
1
Tris-based Protein Extraction Protocol
2
Comprehensive Protein Quantification and Standardization
Check if the same lab product or an alternative is used in the 5 most similar protocols
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All consumables were of electrophoresis grade or higher. Broad-range (7 cm, pH 3-10 non-linear) ReadyStrip TM IPG Strips, Bio-Lyte R carrier ampholyte solutions (pH 3-10; 3-5; 6-8; 7-9; and 8-10), Precision Plus Protein TM Unstained Standards, and tributylphosphine (TBP) were supplied by Bio-Rad Laboratories (Hercules, CA). Isolated protein standards (-galactosidase from E. coli (BGAL); Phosphorylase B from rabbit muscle (PHOSB); Bovine Serum Albumin (BSA); Bovine Carbonic Anhydrase (BCA); and Chicken Egg Lysozyme (CEL)) (Supplementary Table 1), kinase and phosphatase inhibitors (staurosporine, sodium orthovanadate, and sodium fluoride), and components of the protease inhibitor cocktail (PI) [36] were supplied by Sigma-Aldrich (St. Louis, MO). Invitrogen (Carlsbad, CA) supplied the EZQ Protein Quantitation Kit, and all other consumables for electrophoresis and stain preparation, including CBB G-250 dye, and acrylamide/bis-acrylamide (37.5:1) solution, were supplied by Amresco (Solon, OH). Double glass-distilled water (ddH 2 O) was used throughout.
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