This study used the following antibodies for flow cytometry (all from BD): FITC-conjugated anti–annexin V, anti-GL7, and anti-CD49d; PE-conjugated anti-CXCR3, anti-Fas, and anti-CD25; PerCP-Cy5.5–conjugated anti-B220 and anti-CD8; PE-Cy7–conjugated anti-NK1.1, anti-CD11a, and anti-CD45.1; allophycocyanin (APC)-conjugated anti-CD3; and APC-Cy7–conjugated anti-CD4 and anti-CD19. Alexa Fluor 647–conjugated anti-Foxp3 (BD) and PE-conjugated anti-Helios (BioLegend) antibodies were used for intracellular staining of T reg cells. Data were collected using a flow cytometer (LSR II; BD) and analyzed using FlowJo 7.6 software. To deplete NK1.1+ cells, mice were intraperitoneally injected with 1 mg anti-NK1.1 (clone PK136; purified from HB-191 culture supernatants), and persistent NK1.1+ cell depletion for at least 1 mo was confirmed by flow cytometry. For in vivo CXCR3, CTLA-4, or PD-L1 blockade, mice were injected intraperitoneally with 200 µg of functional anti-CXCR3 (CXCR3-173; BioLegend), CTLA-4 (9H10; Bio X cell), or PD-L1 (10F.9G2; BioLegend) every other day.
Pe cy7 conjugated anti nk1
Manufactured by BD
Sourced in United States
PE-Cy7–conjugated anti-NK1.1 is a fluorochrome-labeled antibody that binds to the NK1.1 antigen expressed on natural killer cells in mice. It can be used for the identification and analysis of natural killer cells in flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
Pe conjugated anti cxcr3, by BD (1 mentions)
Percp cy5, by BD (1 mentions)
Fitc conjugated anti annexin 5, by BD (1 mentions)
Alexa fluor 647 conjugated anti foxp3, by BD (1 mentions)
Apc cy7, by BD (1 mentions)
Anti cd11c, by BD (1 mentions)
Anti cd69, by BD (1 mentions)
Apc cy7 conjugated anti cd4, by BD (1 mentions)
Apc conjugated anti f4 80, by Thermo Fisher Scientific (1 mentions)
Percp cy5.5 conjugated anti cd3, by BD (1 mentions)
Fitc conjugated anti ly6c, by BD (1 mentions)
Lsr 2, by BD (1 mentions)
Anti cd11b, by BD (1 mentions)
2 protocols using pe cy7 conjugated anti nk1
1
Multicolor flow cytometry analysis
2
Flow Cytometry Analysis of Immune Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following antibodies were purchased from BD Biosciences (San Jose, CA, USA) and used for flow cytometry analysis: FITC-conjugated anti-Ly6C, anti-CD69, anti-CD11b, and anti–TNF-α; PE-conjugated anti-Ly6G, anti-CD8, and anti–IFN-γ; PerCP-Cy5.5–conjugated anti-CD3; PE-Cy7–conjugated anti-NK1.1 and anti-CD45; allophycocyanin(APC)-conjugated anti-CD45.2, and anti-CD11c; APC-Cy7–conjugated anti-CD4 and anti-CD45.1. APC-conjugated anti-F4/80 were purchased from eBioscience (San Diego, CA, USA). Stained cells were collected using a BD LSR II (BD Biosciences) flow cytometer, and data were analyzed using FlowJo 7.6 software (Tree Star, Ashland, OR, USA). For NK cell depletion, mice were injected with 30 μl anti-ASGM1 antibody once a week (Wako Co., Tokyo, Japan). For NK1.1+cell depletion, mice were intraperitoneally injected with 200 μg anti-NK1.1 (Clone PK136, purified from ascites).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!