PBAT (TH801T) was purchased from Xinjiang Blue Ridge Tunhe Polyester Co., Ltd (Changji, China). It has a specific gravity of 1.21 g/cm3 and a melt mass flow rate (MFR) of 5.0 g/10 min (190 °C), with a number-average molecular weight of 6.12 × 104 g/mol. Reeds were collected from the shore of the lake (Baiyangdian, Baoding, China). For enzymatic degradation, the following enzymes and reagents were used: Proteinase K (≥30 units/mg) from Tritirachium album (Blirt, Gdansk, Poland), esterase (≥10 units/mg) from Bacillus subtilis (Sigma-Aldrich, Bavaria, Germany), lipase (15–25 units/mg) from Candida rugosa (Sigma-Aldrich, Bavaria, Germany), and cellulase (≥200 FPU/mL) from Trichoderma reesei (Novozymes A/S, Copenhagen, Denmark). Tris(hydroxymethyl)aminomethane-HCl (0.1 M, pH = 8.0, Sigma-Aldrich, Germany) and citric acid-sodium citrate (0.1 M, pH = 4.8, Sigma-Aldrich, Germany) were used as buffers for enzyme degradation, sodium azide (NaN3) (Sigma Chemical Co., St. Louis, MI, USA) was used as an antifungal agent, and calcium chloride (Sigma Chemical Co., USA) was used as an activator of enzyme degradation. Other chemical reagents were obtained from Sinopharm Chemical Reagent (Shanghai, China).
Citric acid sodium citrate
Manufactured by Merck Group
Sourced in Germany, United Kingdom
Citric acid-sodium citrate is a buffer solution used to maintain a specific pH range in laboratory applications. It is composed of citric acid and its conjugate base, sodium citrate. The solution helps to stabilize and control the acidity or basicity of the environment, ensuring consistent and reliable experimental conditions.
Automatically generated - may contain errors
Lab products found in correlation
Tritirachium album, by Merck Group (1 mentions)
Bacillus subtilis, by Merck Group (1 mentions)
Candida rugosa, by Merck Group (1 mentions)
Sodium azide nan3, by Merck Group (1 mentions)
Tris hydroxymethyl aminomethane hcl, by Merck Group (1 mentions)
Calcium chloride, by Merck Group (1 mentions)
Proteinase k, by Merck Group (1 mentions)
Coumarin 343, by Merck Group (1 mentions)
Methylene violet, by Merck Group (1 mentions)
6 amino 2 naphthoic acid, by Merck Group (1 mentions)
Biphenyl 4 carboxylic acid, by Merck Group (1 mentions)
4 aminobenzoic acid, by Merck Group (1 mentions)
Cucurbit 7 uril, by Merck Group (1 mentions)
β cyclodextrin, by Merck Group (1 mentions)
2 protocols using citric acid sodium citrate
1
Enzymatic Degradation of Reeds with PBAT
2
Characterization of Fluorescent Probes
Check if the same lab product or an alternative is used in the 5 most similar protocols
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6-amino-2-naphthoic acid (ANA), 4-aminobenzoic acid (ABA), 4′-amino-[1,1′-biphenyl]-4-carboxylic acid (ABPA), methylene violet (Bernthsen) (MV), coumarin 343 (C343), β-cyclodextrin (β-CD), cucurbit[7]uril (CB7), citric acid/sodium citrate, sodium dihydrogen phosphate/disodium hydrogen phosphate, boric acid/sodium borate and sodium N-cyclohexyl-3-aminopropanesulfonate (CAPS) were obtained from Sigma Aldrich UK and were of the highest purity available.
5, 6-diamino-naphthalene-1, 3-disulfonic acid (DANDSA) was obtained from Molekula, UK.
4-pyridinium phenolate (4PP) was synthesised and provided by WuXi App Tec (Hong Kong) Ltd. Structure and purity were confirmed by NMR.
The provenances of Brooker's merocyanine (BM), bis-dimethylaminofuchsone (BDF), phenol blue (PB), and 2, 6-dichloro-4-(2, 4, 6-triphenyl-pyridinium-1-yl)-phenolate (ET33) are as stated previously.
1 (link)
2 (link)
ROW was used throughout this study and had a resistivity of 15MΩcm (checked daily).
Disposable high purity UV-transparent cuvettes (Brand GmbH) with stoppers were used throughout and are described previously.
1 (link)
Disposable four-sided optically transparent fluorescence cuvettes (Brand GmbH) made of the same material as the UV cuvettes, with stoppers, were used to record fluorescence spectra.
5, 6-diamino-naphthalene-1, 3-disulfonic acid (DANDSA) was obtained from Molekula, UK.
4-pyridinium phenolate (4PP) was synthesised and provided by WuXi App Tec (Hong Kong) Ltd. Structure and purity were confirmed by NMR.
The provenances of Brooker's merocyanine (BM), bis-dimethylaminofuchsone (BDF), phenol blue (PB), and 2, 6-dichloro-4-(2, 4, 6-triphenyl-pyridinium-1-yl)-phenolate (ET33) are as stated previously.
1 (link)
2 (link)
ROW was used throughout this study and had a resistivity of 15MΩcm (checked daily).
Disposable high purity UV-transparent cuvettes (Brand GmbH) with stoppers were used throughout and are described previously.
1 (link)
Disposable four-sided optically transparent fluorescence cuvettes (Brand GmbH) made of the same material as the UV cuvettes, with stoppers, were used to record fluorescence spectra.
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