Cell pellets
of Th0 and Th17 cultures (24 and 72 h) were lysed and fractionated
into cytoplasmic and nuclear components using a NE-PER nuclear and
cytoplasmic extraction reagent kit (Thermo Fisher Scientific, cat
no. 78833) by following the manufacturer’s instructions. Extracts
were then analyzed by western blotting. FOSL localization was determined
using anti-FOSL1 (Cell Signaling Tech., cat no. 5281) and anti-FOSL2
(Cell Signaling Tech., cat no. 19967) Ab. Anti-GAPDH (HyTest, cat
no. 5G4) and anti-LSD1 (Diagenode, cat no. C15410067) Ab were used
to mark the cytoplasmic and nuclear fractions, respectively.
of Th0 and Th17 cultures (24 and 72 h) were lysed and fractionated
into cytoplasmic and nuclear components using a NE-PER nuclear and
cytoplasmic extraction reagent kit (Thermo Fisher Scientific, cat
no. 78833) by following the manufacturer’s instructions. Extracts
were then analyzed by western blotting. FOSL localization was determined
using anti-FOSL1 (Cell Signaling Tech., cat no. 5281) and anti-FOSL2
(Cell Signaling Tech., cat no. 19967) Ab. Anti-GAPDH (HyTest, cat
no. 5G4) and anti-LSD1 (Diagenode, cat no. C15410067) Ab were used
to mark the cytoplasmic and nuclear fractions, respectively.