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Paeonol is a bioactive compound extracted from the root bark of the Paeonia plant. It is a laboratory reagent used for research and analysis purposes. Paeonol has a chemical formula of C9H10O3 and a molecular weight of 166.18 g/mol. Its core function is to serve as a reference standard and analytical tool for research applications.

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2 protocols using paeonol

1

Phytochemical-Mediated Anti-Inflammatory Regulation

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Gallic acid, paeonol, and paeoniflorin were purchased from Macklin Biochemical Co., Ltd. (Shanghai, China). Dexamethasone (Dex) was obtained from Sigma Chemical Co. (St Louis, MO, USA). HPLC-grade formic acid was purchased from Meryer Chemical Technology Co., Ltd. (Shanghai, China). All the above chemical reagents were HPLC≥98%. Acetonitrile hypergrade for UPLC-Q/TOF-MS was acquired from Merck KGaA (Darmstadt, Germany). The polyethylenimine (PEI) transient transfection reagent was purchased from PolyScience (Carlsbad, CA, USA). Dual-Luciferase® Reporter Assay System 10-Pack was obtained from Promega (Madison, WI, USA). Both interleukin-6 (IL-6) and interleukin-1 beta (IL-1β) Human ELISA Kit were purchase from Abcam (Cambridge, UK). All reagents for cell culture were obtained from biological industries (Israel).
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2

Melanogenesis Inhibition Screening Assay

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Paeonol (≥98%), tyrosinase (≥500 U mg−1), penicillin–streptomycin solution (100X), l-glutamine (≥99%), and cholesterol (≥99.5%) were purchased from Macklin. Lauric acid (≥99%) was supplied by Alfa Aesar. Phosphate buffered saline (PBS, pH = 6.8, 0.01 M) was purchased from Biorigin. l-Dopa (≥98%) was supplied by Duly Biotech. Co. Ltd (Nanjing, China). Lecithin (from soybean, >90%) was purchased from Aladdin. DMSO (Greagent, ≥99%) and dichloromethane (Greagent, 99.5%) were from Tansoole (Titan Co. Shanghai, China). CCK-8 was from Invigentech. RPMI 1640 cell culture medium and fetal bovine serum (FBS) were supplied by Biological Industries.
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