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Evos fl image system

Manufactured by Thermo Fisher Scientific
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The EVOS FL Image System is a digital inverted fluorescence microscope designed for high-quality imaging of live and fixed cell samples. It features LED illumination, user-friendly software, and a compact design for versatile use in a variety of laboratory settings.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Polybrene, by Thermo Fisher Scientific (1 mentions) Gibco penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Synergy h1 microplate reader, by Agilent Technologies (1 mentions) Nano glo luciferase assay system, by Promega (1 mentions) Rabbit anti ki67 antibody, by Thermo Fisher Scientific (1 mentions) Alexa fluor 555 goat anti rabbit antibody, by Thermo Fisher Scientific (1 mentions) Prolong gold antifade reagent with dapi, by Thermo Fisher Scientific (1 mentions)

4 protocols using evos fl image system

1

SARS-CoV-2 Pseudotyped Lentivirus Neutralization Assay

Cited 1 time
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SARS‑CoV‑2–S Δ19 Pseudotyped Luciferase‑EGFP lentivirus was produced as described previously (Wang et al., 2021 (link)). The 293T/ACE2 target cells were seeded in a 96-well plate (104 cells in 100 µl medium per well) and cultured overnight in a 5 % CO2 incubator at 37 °C. The depleted patient plasma was mixed with the pseudovirus. Each 100 µl of the mixture contained 30 µl depleted plasma, 40 µl pseudovirus, 15 µl DMEM, 5 µl Fetal Bovine Serum (FBS), 1 % Gibco Penicillin-Streptomycin, and 5 µg/ml polybrene (ThermoFisher). After incubating for 1 h at 37 °C, the mixture was added to the cultured 293T/ACE2 cells. The fluorescent images were captured at 72 h post-infection with an EVOS FL Image System (ThermoFisher). The infected 293 T/ACE2 cells were lysed and the luciferase activities were measured using the Nano-Glo Luciferase Assay System (Promega, Madison, WI) and a BioTek SYNERGY H1 microplate reader.
Wang L., Zhao J., Schank M., Khanal S., Dang X., Cao D., Nguyen L.N., Zhang Y., Wu X.Y., Adkins J.L., Brueggeman J., Zhang J., Ning S., El Gazzar M., Moorman J.P, & Yao Z.Q. (2022). Identification of virus-specific B-cell epitopes by convalescent plasma from COVID-19 patients. Molecular Immunology, 152, 215-223.
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2

Fluorescent Visualization of Osteoblast Adhesion

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The adhesion of osteoblasts was also qualitatively and quantitatively assessed by fluorescence, with a cytoskeleton marker used for actin (Actin Probe, Molecular Probes, Carlsbad, CA, USA). Cells were fixed in 4% paraformaldehyde, subjected to permeabilization with 0.1% triton-x and incubated with the actin probe for 30 min in the absence of light. Nuclei were identified by incubation with a DNA intercalant (Hoechst -1:5000) for 15 min. Then, samples were analyzed by fluorescence microscopy (EVOS FL Image System, Thermo Fisher Scientific, Waltham, MA, USA).
Basso F.G., Pansani T.N., Cardoso L.M., Hebling J., Real R.P.V, & Costa C.A.S. (2020). Influence of Bisphosphonates on the Behavior of Osteoblasts Seeded Onto Titanium Discs. Brazilian dental journal, 31(3).
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3

Quantifying SARS-CoV-2 S-mediated Cell Fusion

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Target 293 T cells stably expressing hACE2 (ACE2/293 T, kindly provided by Dr. Hyeryun Choe23 (link)) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with 10% FBS in the presence of 1 µg/ml puromycin. Effector 293 T cells were transiently transfected with pAAV-CMV-Luc-IRES-EGFP-SV40 alone (as negative control), or co-transfected with pAAV-CMV-Luc-IRES-EGFP-SV40 and pCDNA3.1-SARS-S or pCDNA3.1-SARS2-S plasmids (Addgene, Watertown, MA). After 48 h of transfection, the cells were detached with 0.25% Trypsin, and incubated with or without 10% plasma from COVID-19 patients or control subjects at 37 °C for 30 min in 10% FBS-DMEM or 80 ng/ml Trypsin-DMEM and then overlaid on 70–80% confluent ACE2/293 T cells. After co-culturing for 4 h and 24 h, cell fusion images were captured with an EVOS FL Image System (Life Technologies, Frederick, MD) and the numbers of the fused cells within at least 4 randomly selected fields were counted.
Wang L., Zhao J., Nguyen L.N., Adkins J.L., Schank M., Khanal S., Nguyen L.N., Dang X., Cao D., Thakuri B.K., Lu Z., Zhang J., Zhang Y., Wu X.Y., El Gazzar M., Ning S., Moorman J.P, & Yao Z.Q. (2021). Blockade of SARS-CoV-2 spike protein-mediated cell–cell fusion using COVID-19 convalescent plasma. Scientific Reports, 11, 5558.
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4

Quantifying Leukemic Cell Proliferation

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Leukemic cell proliferation was assessed by Ki-67 staining. HL-60 were grown in 6-well plates and treated with 12μM JY-1-106 and 200 nM SR11253 individually or in combination for 48h. After treatments, 10μL of each cell suspension were loaded onto 1 well of 8-well slide coated with poly-L-Lysine. Cells were fixed with 4% paraformaldehyde and permeabilized by 0.1% Triton X-100 for 5 minutes. Slides were blocked with 10% goat serum, incubated with Rabbit anti Ki67 Antibody (Thermo Scientific) overnight in humidified chamber in cold room and then stained with Alexa Fluor® 555 Goat Anti-Rabbit antibody (Invitrogen) for 1 hour at room temperature in dark. One drop of Prolong Gold Antifade Reagent with DAPI (Invitrogen) was applied to each well. Images were taken by EVOS FL image system (Life technologies).
Perri M., Yap J.L., Cione E., Fletcher S, & Kane M.A. (2014). BCL-xL/MCL-1 inhibition and RARγ antagonism work cooperatively in human HL60 leukemia cells. Experimental cell research, 327(2), 183-191.
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