Mouse tnf α kits

Manufactured by Thermo Fisher Scientific

The Mouse TNF-α kits are laboratory reagents used to measure the concentration of tumor necrosis factor-alpha (TNF-α) in mouse biological samples. They provide a quantitative assessment of TNF-α levels, which is an important inflammatory cytokine.

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Lab products found in correlation

Hasmc, by Thermo Fisher Scientific (2 mentions) Oligofectamine, by Thermo Fisher Scientific (2 mentions) Trypsin neutralizer solution, by Thermo Fisher Scientific (2 mentions) Small interfering rna sirna oligonucleotides against rage, by Santa Cruz Biotechnology (2 mentions) Anti hnrnp c1 c2 sc 32308, by Santa Cruz Biotechnology (2 mentions) 2 7 dichlorofluorescein diacetate, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

TNF-α (2204 citations) Mouse TNF-α (43 citations) Mouse TNF-α and IL-1β ELISA kits (4 citations) Mouse TNF-α and IL-6 ELISA kits (4 citations) Mouse TNF-α and mouse IL-6 ELISA kits (2 citations)

2 protocols using mouse tnf α kits

HASMC Culture and Signaling Pathways

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Human SMC growth medium (M231), smooth muscle growth supplement, trypsin/ethylenediaminetetraacetic acid (EDTA) solution, trypsin neutralizer solution, and HASMCs were obtained from Cascade Biologics (Portland, OR). Fetal bovine serum (FBS), antibiotic–antimycotic mixture, mouse TNF-α kits, oligofectamine, and 2’, 7’-dichlorofluorescein diacetate were obtained from Life Technology (Grand Island, NY). Small interfering RNA (siRNA) oligonucleotides against RAGE and antibodies against human TNFR-1 (sc-8436), MSX2 (sc-17729), BMP-2 (sc-6895), RUNX2 (sc-10758), CD68 (sc-9139), β-actin (sc-47778), and anti-hnRNP c1/c2 (sc-32308) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Antibodies against human Nox subunit p47 (#610354), and caveoli-1 (#610406) were obtained from BD Biosciences (San Jose, CA). Unless otherwise specified, all other chemicals and reagents obtained from Sigma-Aldrich (St. Louis, MO). All methods in this study were reported in accordance with ARRIVE guidelines.

Lin C.P., Huang P.H., Chen C.Y., Wu M.Y., Chen J.S., Chen J.W, & Lin S.J. (2021). Sitagliptin attenuates arterial calcification by downregulating oxidative stress-induced receptor for advanced glycation end products in LDLR knockout mice. Scientific Reports, 11, 17851.

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Human Arterial Smooth Muscle Cell Assay

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Human SMC growth medium (M231), smooth muscle growth supplement, trypsin/ethylenediaminetetraacetic acid (EDTA) solution, trypsin neutralizer solution, and HASMCs were obtained from Cascade Biologics (Portland, OR). Fetal bovine serum (FBS), antibiotic-antimycotic mixture, mouse TNF-α kits, oligofectamine, and 2',7'-dichloro uorescein diacetate were obtained from Life Technology (Grand Island, NY). Small interfering RNA (siRNA) oligonucleotides against RAGE and antibodies against human TNFR1 (sc-8436), MSX2 (sc-17729), BMP2 (sc-6895), RUNX2 (sc-10758), CD68 (sc-9139), β-actin (sc-47778), and anti-hnRNP c1/c2 (sc-32308) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Antibodies against human, Nox subunit p47 (#610354), and caveoli-1 (#610406) were obtained from BD Biosciences (San Jose, CA). Unless otherwise speci ed, all other chemicals and reagents obtained from Sigma-Aldrich (St. Louis, MO).

Lin C., Huang P., Chen C., Chen J., Chen J., & Lin S. (2021). Dipeptidyl Peptidase-4 Inhibitor Sitagliptin Attenuates Arterial Calcification By Downregulating Oxidative Stress-Induced Receptor for Advanced Glycation End Products in Low-Density Lipoprotein Receptor Knockout Mice.

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