The chitosan (Mw ~ 370 kDa, DD 85%) and partially N-acetylated chitosan (Mw ~ 200 kDa, DD ~ 50%) were obtained from the Laboratory of Biopolymer Engineering of Institute of Bioengineering of Federal Research Center "Fundamentals of Biotechnology" of Russian Academy of Sciences (Moscow, Russia). Flake crab shell chitin was provided by BioProgress Co. (Shchelkovo, Russia) and grinded in laboratory mill. Colloidal chitin was prepared from powdered crab shell chitin by modi ed method of Rodriguez-Kabana et al. (1983) . Colloidal chitosan was prepared using the modi ed procedure (Helistö et al. 2001 (link)). N-acetyl-β-D-glucosamine, D-glucosamine, p-nitrophenyl-N-acetyl-β-D-glucosaminide (p-NP-GlcNAc), p-nitrophenyl-N,N'-diacetyl-β-D-chitobioside (p-NP-GlcNAc 2 ), p-nitrophenol, sodium salt of carboxymethylcellulose, azokazein, laminarin and xylan from birch were purchased from Sigma Chemical Co., USA. CM-Sepharose Fast Flow was purchased from GE Healthcare (USA). The nutritional components of culture media (tryptone, yeast extract etc.) were obtained from Panreac (Barcelona, Spain) and HiMedia Laboratories Pvt. Ltd. (Mumbai, India). All other reagents, including components for SDS-PAGE, TLC, buffer salts etc. used in the study were quali ed as analytical and high grade.
Azokazein
Manufactured by Merck Group
Sourced in United States
Azokazein is a lab equipment product manufactured by Merck Group. It is a chromogenic substrate used for the detection and quantification of proteolytic enzymes, such as proteases. Azokazein provides a colorimetric signal upon enzymatic cleavage, allowing for the measurement of enzymatic activity.
Automatically generated - may contain errors
Lab products found in correlation
Tryptone, by ITW Reagents (2 mentions)
Yeast extract, by ITW Reagents (2 mentions)
Xylan, by Merck Group (2 mentions)
Laminarin, by Merck Group (2 mentions)
D glucosamine, by Merck Group (2 mentions)
Cm sepharose fast flow, by GE Healthcare (2 mentions)
Sodium salt of carboxymethylcellulose, by Merck Group (2 mentions)
P nitrophenyl n acetyl β d glucosaminide p np glcnac p nitrophenyl n n diacetyl β d chitobioside p np glcnac 2 p nitrophenol, by Merck Group (2 mentions)
N acetyl β d glucosamine, by Merck Group (2 mentions)
2 protocols using azokazein
1
Chitosan and Chitin Preparation Protocol
2
Chitosan and Chitin Preparation Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The chitosan (Mw ~ 370 kDa, DD 85%) and partially N-acetylated chitosan (Mw ~ 200 kDa, DD ~ 50%) were obtained from the Laboratory of Biopolymer Engineering of Institute of Bioengineering of Federal Research Center "Fundamentals of Biotechnology" of Russian Academy of Sciences (Moscow, Russia). Flake crab shell chitin was provided by BioProgress Co. (Shchelkovo, Russia) and grinded in laboratory mill. Colloidal chitin was prepared from powdered crab shell chitin by modi ed method of Rodriguez-Kabana et al. (1983) . Colloidal chitosan was prepared using the modi ed procedure (Helistö et al. 2001 (link)). N-acetyl-β-D-glucosamine, D-glucosamine, p-nitrophenyl-N-acetyl-β-D-glucosaminide (p-NP-GlcNAc), p-nitrophenyl-N,N'-diacetyl-β-D-chitobioside (p-NP-GlcNAc 2 ), p-nitrophenol, sodium salt of carboxymethylcellulose, azokazein, laminarin and xylan from birch were purchased from Sigma Chemical Co., USA. CM-Sepharose Fast Flow was purchased from GE Healthcare (USA). The nutritional components of culture media (tryptone, yeast extract etc.) were obtained from Panreac (Barcelona, Spain) and HiMedia Laboratories Pvt. Ltd. (Mumbai, India). All other reagents, including components for SDS-PAGE, TLC, buffer salts etc. used in the study were quali ed as analytical and high grade.
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