Mab348

Antibodies to the PS1 CTF (3027 47, immunoblot (IB): 1:4,000), the PS2 CTF (BI.HF5c 23, IB: 1:2,000) as well as antibodies to total Aβ (3552 48, immunoprecipitation (IP) 1:500 and 2D8 49, IB 3 μg/ml) have been described previously. Monoclonal antibody 2G7 (IgG2b/k) (IB 3 μg/ml) to the PS1 NTF was raised in C57/BL6 mice against residues 39–52 (NDRRSLGHPEPLSN) of human PS1. Antibodies N1660 to NCT (Sigma N1660, IB 1:1,000), 22C11 to secreted soluble APP (Merck Millipore MAB348, IB 1:5,000), 4G8 to Aβ (Covance SIG‐39220, IP 1:500–1:2,500), Y188 to the APP C‐terminus (Abcam ab32136, IB 1:4,000), and Penta‐His (Qiagen 34460, IB 1:2,000) were obtained from the indicated companies. Species‐specific anti‐Aβ antibodies to Aβ40 (BAP24) and Aβ42 (BAP15) were kindly provided by Manfred Brockhaus (Roche Applied Science) and SULFO‐tagged according to the instructions of the supplier (Meso Scale Discovery (MSD)). The anti‐Aβ37 antibody (D2A6H) was obtained from Cell Signaling (#12467S) and SULFO‐tagged as above. The SULFO‐tagged antibody against Aβ38 was obtained from MSD.

Antibodies to PS1 N‐terminal (PS1N, Capell et al, 1997, immunoblot (IB) 1:1,000) and PS2 C‐terminal fragments (BI.HF5c, Steiner et al, 1999, IB: 1:2,000), respectively, as well as antibodies to the C‐terminus of APP (6687, Steiner et al, 2000, IB 1:1,000), and to total Aβ (3552, Yamasaki et al, 2006, immunoprecipitation (IP) 1:500–1:7,500 and 2D8, Shirotani et al, 2007, IB 3 μg/ml), have been described previously. Neoepitope‐specific antibody to Val50 of AICD (IB 5 μg/ml) was a gift from Eli Lilly and Company and has been described before (Chavez‐Gutierrez et al, 2012). End‐specific antibodies to Aβ40, Aβ42, and Aβ43 characterized previously (Saito et al, 2011) were obtained from IBL (JP18580, JP18582, and JP 18583, immunohistochemistry (IHC) 2 μg/ml). Antibodies NT1 to the PS1 NTF (Covance SIG‐39194, IB 1:2,000), N1660 to NCT (Sigma N1660, IB 1:1,000), 22C11 to secreted soluble APP (Merck Millipore MAB348, IB 1:5,000), 4G8 to Aβ (Covance SIG‐39220, IP 1:500–1:2,500; and BioLegend 800701, IHC 1:500), Y188 to the APP C‐terminus (Abcam ab32136, IB 1:4,000), Penta‐His (Qiagen 34460, IB 1:2,000), and AT‐8 to hyperphosphorylated microtubule‐associated protein tau (Thermo Scientific MN1020, IHC 1:200) were obtained from the indicated companies. Rat monoclonal antibody 5E12 (IB 2.5 μg/ml) of IgG2a subclass was raised against residues 313‐333 (SKYNAESTERESQDTVAENDD) of human PS1.