N oleoyl d erythro sphingosylphosphorylcholine

1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine (PLPC) and n-oleoyl-d-erythro-sphingosylphosphorylcholine (SM(d18:1/18:1)) were obtained from Avanti Polar Lipids (AL, USA). Chloroform was purchased from Merck (Darmstadt, Germany). Water was purified (resistance > 18 mΩ/cm) on a PureLab Ultra Analytic System (ELGA Lab Water, Celle, Germany). Ammonium bicarbonate, ammonium formate, sodium ascorbate, and copper (II) sulfate anhydrous were purchased from Sigma-Aldrich (Munich, Germany). UPLC-grade methanol, acetonitrile, formic acid, and isopropanol were obtained from Biosolve VB (Valkenswaard, The Netherlands).

1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine (PLPC) and n-oleoyl-d-erythro sphingosylphosphorylcholine (SM (d18:1/18:1)) were obtained from Avanti Polar Lipids (Alabaster, AL, USA). Methyl-tert-buthylether (MTBE) and chloroform were purchased from Sigma Aldrich (Taufkirchen, Germany). Potassium ferrocyanide (K₄[Fe(CN)₆]), disodium hydrogen phosphate (Na₂HPO₄), and sodium dihydrogen phosphate (NaH₂PO₄) were obtained from Merck (Darmstadt, Germany). Sodium sulfate was purchased from VWR (Darmstadt, Germany). UPLC-grade methanol, acetonitrile, formic acid, and isopropanol were obtained from Biosolve BV (Valkenswaard, Netherlands). Hydrogen peroxide (30%) and 32% ammonia solutions are from Carl Roth (Karlsruhe, Germany). 50 mM buffer solution of (Na₂HPO₄/NaH₂PO₄) (pH = 7.4) was used in the experiments. All chemicals employed were used without further purification. In all solutions and experiments ultrapure water was used (resistivity: 18.2 MΩ.cm, conductivity: 0.055 μS/cm) prepared by Thermo Scientific GenPure pro water purification system (Niederelbert, Germany).