Egg phosphatidylcholine epc

The ¹⁹F-labeled octaarginine (¹⁹F-R8: (4CF₃-Phe)-RRRRRRRR) was synthesized manually by solid phase synthesis method using Fmoc chemistry. The amino and carboxyl termini of the peptide were acetylated and amidated, respectively. A fragment peptide, called ¹⁹F-T6 (TV-(4CF₃-Phe)-DSGISEVR), from human lens αA-crystallin [69 (link)] was used as a negative control and synthesized by Fmoc solid-phase chemistry using an automated solid-phase synthesizer (PSSM-8; Shimadzu, Kyoto, Japan). The purity of each peptide was confirmed to be > 95% by reversed-phase high-performance liquid chromatography and MALDI-TOF mass spectrometry. Heparin sodium salt (from porcine intestinal mucosa; average molecular weight, 18,000 Da) was purchased from SIGMA (St. Louis, MO, USA). Egg phosphatidylcholine (EPC, > 96% pure) and egg phosphatidylglycerol (EPG, > 95% pure) were obtained from the NOF CORPORATION (Tokyo, Japan). All other reagents were of special grade and used without further purification.