A cell viability assay was performed using the EZ-Cytox Enhance cell viability assay kit (DoGenBio, Seoul, Republic of Korea). The HDPCs were seeded in 96-well plates and incubated for 24 h. Cells were irradiated for 0, 30, 60, and 120 min with total radiant exposure 0, 5.4, 10.8, 21.6 J/cm2, and cell viability was examined following the protocol below. Briefly, 100 µL EZ-Cytox solution was added to each well and incubated in a 5% CO2 incubator for 1.5 h at 37 °C. Optical density (OD) values of samples were measured at 450 nm using a microplate reader (BioTek, El Segundo, CA, USA).
Ez cytox enhance cell viability assay kit
Manufactured by DoGenBio
The EZ-Cytox Enhance cell viability assay kit is a laboratory product designed to measure cell viability. It provides a quantitative method to assess the metabolic activity of cells in culture.
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Lab products found in correlation
2 protocols using ez cytox enhance cell viability assay kit
1
Cell Viability Assay of HDPC
2
Macrophage Viability and NO Assay
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BMDMs were pretreated with the indicated concentrations of cpTLR-i and activated with 200 ng/ml LPS for 24 h. Cell viability was measured using the EZ-Cytox Enhance Cell Viability Assay Kit (DoGenBio) in accordance to the manufacturer’s instruction. Production of nitrogen oxide (NO) in the culture supernatant was determined by the Griess Reagent System (Promega).
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