The soil samples were sieved through a 2-mm mesh to remove roots and then ground and homogenized with a mill (MM400, Retsch, Germany). 15 g of fresh soil was weighed before and after dried at 105 °C for 24 h to calculate the soil water content (SWC). The soils were subjected to Kjeldahl digestion, and the soil total nitrogen (TN) concentration was determined using a semi-autoanalyzer (
Kjeltec 2300 Analyzer Unit, Foss Tecator, Sweden). Soil total phosphorus (TP) concentration was measured by persulfate oxidation followed by colorimetric analysis (Schade et al., 2003 (
link)). Soil total organic carbon (TOC) was determined with a
TOC-5000A analyzer (Shimadzu Corp., Kyoto, Japan). Soil pH was measured using a ratio of soil:water = 1g:2.5 ml with a
Delta pH-meter (Mettler-Toledo Instruments, Columbus, OH, USA).
Chen L., Shi J., Bao Z, & Baoyin T. (2020). Soil fungal networks are more sensitive to grazing exclusion than bacterial networks. PeerJ, 8, e9986.