Diagnostic assay kit

Manufactured by Nanjing Jiancheng

Sourced in China

The Diagnostic assay kit is a laboratory equipment product designed for conducting various diagnostic tests. It provides the necessary reagents, materials, and protocols to perform specific analyses. The core function of the assay kit is to facilitate accurate and reliable diagnostic procedures.

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Lab products found in correlation

Du 640 spectrophotometer, by Beckman Coulter (1 mentions) Anti rhoa, by Cell Signaling Technology (1 mentions) Anti α tubulin antibody, by Cell Signaling Technology (1 mentions) Anti rock, by Cell Signaling Technology (1 mentions) Simvastatin sim, by Merck Group (1 mentions) Palmitic acid, by Merck Group (1 mentions) Oleic acid, by Merck Group (1 mentions) Creatinine, by Nanjing Jiancheng (1 mentions) Cell counting kit 8 cck 8, by Nanjing Jiancheng (1 mentions)

4 protocols using diagnostic assay kit

Nitric Oxide Detection in MI Tissue

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The level of nitric oxide (NO) in MI cardiac tissue was detected using a diagnostic assay kit (Nanjing Jiancheng Bioengineering Research Institute, Nanjing, China) as described in a previous study. 18 (link)

Wang Y., Zhang S., Ma Y., Xiang A., Sun H., Song J., Yang W., Li X, & Xu H. (2022). Melatonin protected against myocardial infarction injury in rats through a Sirt6-dependent antioxidant pathway. Advances in clinical and experimental medicine : official organ Wroclaw Medical University, 31(3).

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Quantifying Cardiac Nitric Oxide Levels

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Following reperfusion, the hearts were removed from the Langendorff device and homogenized in ice-cold 0.9% saline solution, and then centrifuged at 600 × g for 10 min. The NO and NOS levels were measured using a diagnostic assay kit (Nanjing Jiancheng Bioengineering Research Institute). The absorbance was determined using a DU-640 spectrophotometer (Beckman Coulter Inc., Brea, CA, USA) at 530 nm and plotted as a percentage of the control according to the manufacturer's instructions.

CAO J., XIE H., SUN Y., ZHU J., YING M., QIAO S., SHAO Q., WU H, & WANG C. (2015). Sevoflurane post-conditioning reduces rat myocardial ischemia reperfusion injury through an increase in NOS and a decrease in phopshorylated NHE1 levels. International Journal of Molecular Medicine, 36(6), 1529-1537.

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Visceral Adiposity and Metabolic Markers

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Body weight was recorded twice a week throughout the experiment. All animals were sacrificed by decapitation 6 h after fasting. Blood samples were collected and centrifuged at 4,000 rpm for 15 min at 4℃. Visceral adipocyte tissues (white fat tissues) were dissected immediately, rinsed with cold physiologic saline solution, weighed, frozen in liquid nitrogen and stored at −80℃ for further analysis. Glycerol, TG, and TC were respectively detected via diagnostic assay kits from Nanjing Jiancheng Company following the manufacture's protocol.

Yu W.G., He Y., Chen Y.F., Gao X.Y., Ning W.E., Liu C.Y., Tang T.F., Liu Q, & Huang X.C. (2019). Fumigaclavine C attenuates adipogenesis in 3T3-L1 adipocytes and ameliorates lipid accumulation in high-fat diet-induced obese mice. The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology, 23(3), 161-169.

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Endophytic Aspergillus terreus Derived Anti-RhoA Compounds

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FC (≥ 99.5%) was collected from endophytic Aspergillus terreus (strain No. FC118) via the root of Rhizophora stylosa (Rhizophoraceae). The anti-RhoA, anti-prenyl, anti-ROCK, anti-sterol response element binding protein-1c (Srebp-1c), anti-α-tubulin antibodies, and secondary antibody were purchased from Cell Signaling Technology (Danvers, MA, USA). Oleic acid, palmitic acid, and simvastatin (Sim) was purchased from Sigma Aldrich Company (St. Louis, MO, USA). Total cholesterol (TC), Triglyceride (TG), low density lipoprotein-cholesterol (LDL-c), high density lipoprotein-cholesterol (HDL-c), free fatty acid (FFA), total bilirubin (T-bili), alanine transaminase (ALT), aspartate transaminase (AST), creatinine, creatine kinase, and Cell Counting Kit-8 (CCK-8) were measured by diagnostic assay kits from Nanjing Jiancheng Company (NJ, China).

Yu W., Gao Y., Zhao Z., Long X., Yi Y, & Ai S. (2023). Fumigaclavine C ameliorates liver steatosis by attenuating hepatic de novo lipogenesis via modulation of the RhoA/ROCK signaling pathway. BMC Complementary Medicine and Therapies, 23, 288.

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