H1975, H820, H1299 NSCLC cell lines and CCD-19Lu normal lung fibroblast cell line were purchased from American Type Culture Collection (ATCC) (Manassas, VA, USA). The human normal lung fibroblast CCD-19Lu cells were grown in monolayer culture in MEM medium, whereas the three NSCLC cell lines were all grown in monolayer culture in RPMI 1640 medium. All culture media were supplemented with 10% FBS with 100 U/ml penicillin and 100 g/ml streptomycin. All cells were cultivated at 37 °C in a humidified atmosphere of 5 % CO2.
H1299 nsclc cell lines
Manufactured by American Type Culture Collection
Sourced in United States
The H1299 NSCLC cell line is a non-small cell lung cancer (NSCLC) cell line derived from a lymph node metastasis. It is a commonly used model for studying NSCLC biology and exploring potential therapeutic approaches.
Automatically generated - may contain errors
Lab products found in correlation
H1975, by American Type Culture Collection (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Antibiotic antimycotic, by Thermo Fisher Scientific (1 mentions)
Trypsin edta solution, by Thermo Fisher Scientific (1 mentions)
2 protocols using h1299 nsclc cell lines
1
Cell Line Culture and Maintenance Protocols
2
Culturing NSCLC and Fibroblast Cell Lines
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A549, H460 and H1299 NSCLC cell lines were obtained from the American Type Culture Collection (ATCC). The human dermal fibroblasts were obtained from human foreskin (IRB#100–05-251) [55] . All types of NSCLC cells were grown in the Roswell Park Memorial Institute medium (RPMI 1640, Gibco, USA), while the human fibroblasts were cultured in the high-glucose Dulbecco’s modified Eagle medium (DMEM, Gibco, USA). The culture medium contains 10% fetal bovine serum (FBS, Gibco, USA) and 10 mg/L Antibiotic-Antimycotic (Gibco, USA). Cells were maintained in a humidified 5% CO2 chamber at 37 °C. The cultured cells were resuspended using 0.25% trypsin/EDTA solution (Gibco) every 3 days for subculture process. The medium replacement was performed two times every week.
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