CAPE procured from Sigma-Aldrich was dissolved in sterile dimethyl sulfoxide (DMSO) diluted with isotonic saline (1:5). STZ was procured from Sisco Research Laboratories Pvt. Ltd., Mumbai. Dihydrogen orthophosphate, 5,5’-dithiobis (2-nitrobenzoic acid) (DTNB) (Himedia Laboratories, Mumbai); thiobarbituric acid, trichloroacetic acid, sodium dodecyl sulphate (Loba Chemie, Mumbai); and TNF-α ELISA kit (Krishgen, Mumbai) were used. Artificial cerebrospinal fluid (aCSF) was prepared as follows: (in mmol/L) 147 mM NaCl, 2.9 mM KCl, 1.6 mM MgCl2, 1.7 mM CaCl2, 2.2 mM dextrose were dissolved in 10 mL of water for injection.[9 (link)] All the drug solutions were freshly prepared before injections.
Trichloroacetic acid
Manufactured by Loba Chemie
Sourced in India
Trichloroacetic acid is a colorless, crystalline solid chemical compound with the formula CCl3COOH. It is a widely used reagent in various analytical and organic chemistry applications.
Automatically generated - may contain errors
Lab products found in correlation
Thiobarbituric acid, by Loba Chemie (2 mentions)
Sodium dodecyl sulphate (sds), by Loba Chemie (1 mentions)
Streptozotocin (stz), by Sisco Research Laboratories (1 mentions)
Milli q system, by Merck Group (1 mentions)
Hydrogen peroxide, by Loba Chemie (1 mentions)
Pentylenetetrazol, by Merck Group (1 mentions)
Butylated hydroxyl toluene, by Loba Chemie (1 mentions)
1 1 diphenyl 2 picrylhydrazyl dpph, by HiMedia (1 mentions)
3 protocols using trichloroacetic acid
1
CAPE and STZ-Induced Neuroinflammation
2
Casein Glycomacropeptide Detection in Milk
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Trichloroacetic acid, monopotassium phosphate, and dipotassium phosphate were purchased from Loba Chemie Pvt (Mumbai, India). Sodium sulfate was obtained from Schaurlau (Barcelona, Spain). Deionized water was purified using a Milli-Q system (Millipore; Bedford, MA, USA). Rennet (chymosin) enzyme was supplied by the CAL Group (Bogota, Colombia). A cGMP pattern was acquired from Sigma-Aldrich (St. Louis, MO, USA. An immunochromatographic test for casein glycomacropeptide detection in milk (Stick cGMP) was acquired from Operon (Zaragoza, Spain).
3
Antioxidant Activity Evaluation Protocol
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Pentylenetetrazol (dissolved in normal saline) was obtained from Sigma-Aldrich (St. Louis, MO). Ammonium thiocyanate, butylated hydroxyl anisole, thiobarbituric acid, butylated hydroxyl toluene, sulfanilamide, a-tocopherol, pyrocatechol, trichloroacetic acid, hydrogen peroxide, 5,5 0 -dithiobis-(2nitrobenzoic acid), reduced glutathione (GSH), bovine serum albumin (BSA) from Loba Chemie (Mumbai, India). 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) from Himedia Chemicals (Mumbai, India). All other reagents employed in the study were of analytical grade. Phenytoin injection was obtained locally from Cadila Laboratories (Ahmedabad, India).
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