Extracted samples were analyzed with a UPLC Waters Acquity system equipped with a Waters TQ-S micro mass spectrometer (triple quadrupole detector) added to an electrospray ionization source (ESI). Components were eluted in a linear gradient with sequential changes in the proportion of aqueous eluent (ammonium formate in ultrapure water, pH = 3.2) and organic eluent (acetonitrile with formic acid 0.1% [v/v]), as previously described [6 (link)]. Data were analyzed with MassLynx software (version 4.2, Waters, Milford, MA, USA). The multiple reaction monitoring (MRM) mode was used for mass spectrometry detection. Selected MRM characteristic transitions were 438.0 > 357.0 (loss of SO2NH2) for pazopanib, 452.0 > 328.0 (loss of SO2NH2, CHO and CH3) and 452.0 > 343.0 (loss of SO2NH2 and CHO) for P-CHO. S-CHO was detected with 413.1 > 340.1 (loss of N(CH2CH3)2), 413.1 > 297.1 (loss of N(CH2CH3)2-(CH2)2-NH-CO), and 413.1 > 268.1 (cleavage of the amide bond and loss of CHO).
Tq s micro mass spectrometer
Manufactured by Waters Corporation
The TQ-S Micro mass spectrometer is a compact and sensitive instrument designed for a range of analytical applications. It features a triple quadrupole configuration and is capable of performing high-resolution mass analysis and tandem mass spectrometry (MS/MS) experiments.
Automatically generated - may contain errors
Lab products found in correlation
Ace excel c18 ar column, by Waters Corporation (2 mentions)
Acquity uplc system, by Waters Corporation (2 mentions)
Acquity system, by Waters Corporation (1 mentions)
Masslynx software, by Waters Corporation (1 mentions)
Tacrine, by Merck Group (1 mentions)
Midazolam, by Merck Group (1 mentions)
Bufuralol, by Merck Group (1 mentions)
Diclofenac, by Merck Group (1 mentions)
Acquity uplc ms ms system, by Waters Corporation (1 mentions)
Acquity binary solvent manager, by Waters Corporation (1 mentions)
4 protocols using tq s micro mass spectrometer
1
UPLC-MS/MS Analysis of Pazopanib and Metabolites
2
Multiplexed CYP Enzyme Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To determine the activities of CYP -1A2, -2C9, -2D6 and -3A4 respectively, the conversion of Tacrine (5 μmol) to 1-hydroxyTacrine, Diclofenac (90 μmol) to 4-hydroxydiclodenac, Bufuralol (10 μmol) to hydroxyBufuralol and Midazolam (5 μmol) to 1-hydroxyMidazolam were analysed (compounds supplied by Sigma Aldrich, United Kingdom). A mixture of the four substrates was prepared at 1000-fold concentration in DMSO and added to microtissues during a full medium change. Cells were incubated for 2 h under standard culture conditions. Metabolites were quantified in cell culture medium by liquid chromatography tandem mass spectrometry. Samples were separated using a Waters Acquity UPLC system with an ACE Excel C18-AR column and analysed on a Waters TQ-S Micro mass spectrometer against quantitative standard curves.
3
UPLC-MS/MS for Analytical Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A Waters Acquity UPLC–MS/MS system (Waters Corp., Milford, MA, USA) consisting of an Acquity binary solvent manager (chromatographic pump), a sample manager (autosampler) and a column manager was used. The UPLC was connected to a Waters TQ‐S micro mass spectrometer with a triple quadrupole. The software programs Masslynx™ V4.1 and Targetlynx V4.1 were used for data processing.
4
Compound Metabolic Turnover and Protein Binding
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To determine the metabolic turnover of obeticholic acid and elafibranor in the MPS platform, both compounds were incubated with liver microtissues for 48 h starting at 1 µM and media samples taken at regular intervals (AdooQ Bioscience, UK). The presence of each compound in the cell culture medium was quantified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Samples were separated using a Waters Acquity UPLC system with an ACE Excel C18-AR column and analysed on a Waters TQ-S Micro mass spectrometer against quantitative standard curves. Both compounds were also assessed for protein binding in HEP-Fat media using rapid equilibrium dialysis (RED) and quantified using the same LC-MS/MS methodology.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!