The Wantai BioPharma (Beijing, China) ELISA was used for the detection of immunoglobulins IgM or total Ig of SARS-CoV-2 RBD, and Roche immunoassay was used to examine anti-S total Ig (Elecsys anti-SARS-CoV-2 S (RBD)) (Roche, Basel, Switzerland). The GenScript surrogate virus neutralization test (sVNT) (Piscataway, NJ, USA) was used to detect the presence of antibodies interfering with RBD binding to the ACE2 receptor. For the GenScript sVNT assay, 30 to <60% was regarded as low, 60% to 90% as medium, and >90% as high levels of potentially neutralizing antibodies. The tests were performed according to the manufacturer’s instructions (https://www.genscript.com/covid-19-detection-svnt.html ) (accessed on 26 May 2023) and as described by Tan et al. [34 (link)].
Elecsys anti sars cov 2 s rbd
Manufactured by Roche
Sourced in Switzerland
The Elecsys anti-SARS-CoV-2 S (RBD) is a quantitative electrochemiluminescence immunoassay (ECLIA) used for the in vitro determination of antibodies to the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein in human serum and plasma.
Automatically generated - may contain errors
3 protocols using elecsys anti sars cov 2 s rbd
1
SARS-CoV-2 Antibody Detection Protocols
2
SARS-CoV-2 Antibody Detection in DBS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
DBS specimens were tested using the Elecsys Anti-SARS-CoV-2 (nucleocapsid) and Elecsys Anti-SARS-CoV-2 S (RBD) assays (Roche Diagnostics, Basel, Switzerland) at the National Microbiology Laboratory (NML; Public Health Agency of Canada, Winnipeg, Canada) according to the manufacturer’s instructions. DBS samples were punched (4 × 6 mm) using a semi-automated BSD600 Ascent puncher (BSD Robotics) into 2 mL 96-well polypropylene plates (ThermoFisher Scientific). DBS punches were eluted in 370 μL of DPBS containing 0.5% BSA and 0.05% Tween 20 overnight at 4 °C with agitation (400 RPM). Afterwards, plates were incubated at room temperature for 30 min with agitation (400 RPM) and 250 μL of DBS eluate was transferred into 2 mL microtubes for direct loading onto a cobas e 411 analyser (Roche Diagnostics). A result of <0.8 U/mL and ≥0.8 U/mL was interpreted as negative and positive respectively with the Elecsys Anti-SARS-CoV-2 S. A cutoff index <1.0 and ≥1.0 was interpreted as negative and positive respectively with the Elecsys Anti-SARS-CoV-2 assay. DBS punching and elution protocols were validated using an earlier DBS panel [9 ].
3
SARS-CoV-2 Antibodies and T-Cell Responses in PLWH
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood samples were collected at baseline and on day 35. Elecsys® Anti-SARS-CoV-2 S RBD (Roche Diagnostics) was used to identify and quantify antibodies specific to SARS-CoV-2 spike protein in serum samples84 . Spike-specific CD4+ T-cell responses were quantified using activation-induced marker assays via up-regulation of CD69 and CD40L (CD154), as previously described85 (link). Total IgG levels were analyzed by routine diagnostic methods with the Roche Elecsys anti-SARS-CoV-2 S enzyme immunoassay, at the Clinical Immunology laboratory of Karolinska University Hospital84 . For PLWH, CD4+ and CD8+ T-cell counts and HIV viral load (VL) were determined by flow cytometry and Cobas Amplicor (Roche Molecular Systems Inc., USA), respectively86 (link).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!