STX-S or 293F parental exosomes were mixed with anti-CD81-labeled magnetic beads for 2 h at RT (catalog number 10622D; Thermo Fisher) and washed twice with PBS using a magnetic stand. Next, the bead-exosome mixtures were incubated with either directly Alexa Fluor 647-conjugated antispike antibody (see the section on flow cytometry, above), fluorescein isothiocyanate (FITC)-conjugated anti-CD81 antibody (catalog number 551108; BD Biosciences), or FITC-conjugated mouse IgGκ isotype control antibody (catalog number 555748; BD Biosciences) for 1 h at RT, followed by two PBS washes. 293F exosomes were used as a negative control for spike expression, and the isotype antibody was used as a negative control for CD81 expression. Samples were analyzed on a CytoFlex S flow cytometer (Beckman Coulter), and data were analyzed by using FlowJo (Table S2).
Fluorescein isothiocyanate fitc conjugated anti cd81 antibody
Manufactured by BD
Fluorescein isothiocyanate (FITC)-conjugated anti-CD81 antibody is a laboratory reagent used to detect and analyze the presence of CD81 proteins in biological samples. The antibody is labeled with the fluorescent dye FITC, which allows the detection and quantification of CD81-expressing cells through techniques such as flow cytometry.
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2 protocols using fluorescein isothiocyanate fitc conjugated anti cd81 antibody
1
Exosome Surface Protein Analysis
2
Exosome Surface Protein Analysis
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STX-S or 293F parental exosomes were mixed with anti-CD81-labeled magnetic beads for 2 h at RT (catalog number 10622D; Thermo Fisher) and washed twice with PBS using a magnetic stand. Next, the bead-exosome mixtures were incubated with either directly Alexa Fluor 647-conjugated antispike antibody (see the section on flow cytometry, above), fluorescein isothiocyanate (FITC)-conjugated anti-CD81 antibody (catalog number 551108; BD Biosciences), or FITC-conjugated mouse IgGκ isotype control antibody (catalog number 555748; BD Biosciences) for 1 h at RT, followed by two PBS washes. 293F exosomes were used as a negative control for spike expression, and the isotype antibody was used as a negative control for CD81 expression. Samples were analyzed on a CytoFlex S flow cytometer (Beckman Coulter), and data were analyzed by using FlowJo (Table S2).
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